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P3296

Millipore

Protein G Sepharose™, Fast Flow

recombinant, expressed in E. coli, aqueous ethanol suspension

Synonim(y):

Protein G-Agarose, Fast Flow from Streptococcus sp.

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About This Item

Numer MDL:
MFCD00165580
Kod UNSPSC:
41106500
NACRES:
NA.56

rekombinowane

expressed in E. coli

Postać

aqueous ethanol suspension

klasy chemiczne analitów

proteins (Immunoglobulins of various mammalian species)

zakres etykietowania

~2 mg per mL

metody

affinity chromatography: suitable

macierz

Sepharose 4B Fast Flow

aktywacja macierzy

cyanogen bromide

MAR

amino

spacer macierzy

1 atom

temp. przechowywania

2-8°C

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Opis ogólny

Protein G is an immunoglobulin (IgG)-binding bacterial cell wall protein isolated from group G streptococcal strain, G-148. This protein can be extracted from the cells by papain digestion and purified by the sequential use of ion-exchange chromatography on DEAE-Sephadex, affinity chromatography on Sepharose-coupled human IgG, and gel chromatography on Sephadex G-200. The binding between protein G and various polyclonal and monoclonal IgG is basically pH dependent between 2.8 and 10, with the strongest binding at pH 4 and 5, and weakest at pH 10. It acts as a powerful reagent for the detection of IgG.
Protein G is an immunoglobulin (IgG)-binding bacterial cell wall protein isolated from group G streptococcal strain, G-148. This protein can be extracted from the cells by papain digestion and purified by the sequential use of ion-exchange chromatography on DEAE-Sephadex, affinity chromatography on Sepharose-coupled human IgG, and gel chromatography on Sephadex G-200. The binding between protein G and various polyclonal and monoclonal IgG is basically pH dependent between 2.8 and 10, with the strongest binding at pH 4 and 5, and weakest at pH 10. It acts as a powerful reagent for the detection of IgG.

P3296-5Ml′s updated product number is GE17-0618-01

Zastosowanie

Protein G-Sepharose™ is used in affinity chromatography, protein chromatography, antibody purification and characterization, immunoaffinity matrices, protein A, G and L resins, protein interaction, and purification and detection. Protein G-Sepharose™ has been used to develop a strategy to confirm the presence of anti-erythropoietin neutralizing antibodies in human serum as well as to compare methods for depletion of albumin and IgG from equine serum.

Postać fizyczna

Suspension in 20% ethanol

Uwaga dotycząca przygotowania

Prepared with recombinant streptococcal Protein G from which the albumin-binding region has been genetically deleted

Informacje prawne

Sepharose is a trademark of Cytiva

Piktogramy

Flame
GHS02

Hasło ostrzegawcze

Warning

Zwroty wskazujące rodzaj zagrożenia

H226

Zwroty wskazujące środki ostrożności

P210 - P233 - P240 - P241 - P242 - P243

Klasyfikacja zagrożeń

Flam. Liq. 3

Kod klasy składowania

3 - Flammable liquids

Klasa zagrożenia wodnego (WGK)

WGK 3

Temperatura zapłonu (°F)

115.0 °F - closed cup

Temperatura zapłonu (°C)

46.1 °C - closed cup

Certyfikaty analizy (CoA)

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Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

G Yang et al.
Oncogene, 26(1), 91-101 (2006-06-27)
The t(8;21) chromosomal translocation that generates the fusion oncoprotein RUNX1-ETO predominates in leukemia patients of the French-American-British (FAB) class M2 subtype. The oncoprotein has the capacity to promote expansion of hematopoietic stem/progenitor cells and induces leukemia in association with other
B Akerström et al.
Journal of immunology (Baltimore, Md. : 1950), 135(4), 2589-2592 (1985-10-01)
Protein G is an immunoglobulin (IgG)-binding bacterial cell wall protein recently isolated from group G streptococci. We have investigated the avidity of protein G for various monoclonal and polyclonal Ig of the IgG class, and compared it with the binding
Yi-Jye Chern et al.
Cell death & disease, 10(7), 504-504 (2019-06-28)
Therapy-refractory disease is one of the main contributors of treatment failure in cancer. In colorectal cancer (CRC), SPARC can function as a sensitizer to conventional chemotherapy by enhancing apoptosis by interfering with the activity of Bcl-2. Here, we examine a
Kyle A Nilson et al.
Nucleic acids research, 45(19), 11088-11105 (2017-10-05)
Oxidative stress has pervasive effects on cells but how they respond transcriptionally upon the initial insult is incompletely understood. We developed a nuclear walk-on assay that semi-globally quantifies nascent transcripts in promoter-proximal paused RNA polymerase II (Pol II). Using this
Rajesh P Ringe et al.
Journal of virology, 94(6) (2019-12-20)
We covalently attached human immunodeficiency virus type 1 (HIV-1) Env SOSIP trimers to iron oxide nanoparticles (IO-NPs) to create a particulate immunogen for neutralizing antibody (NAb) induction. The attached trimers, ∼20 per particle, retained native-like antigenicity, judged by reactivity with
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Protokoły

Immunoprecipitation Procedure

Techniques for protein antigen molecular weight determination, protein interactions, enzymatic activity, and post-translational modifications.

Procedura immunoprecypitacji

Techniki określania masy cząsteczkowej antygenu białkowego, interakcji białkowych, aktywności enzymatycznej i modyfikacji potranslacyjnych.

Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.

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