N3393

Naphthol Blue Black

BioReagent, suitable for electrophoresis

• Synonim(y): Acid Black 1, Amido Black 10B, Buffalo Black NBR
• Wzór empiryczny (zapis Hilla): C₂₂H₁₄N₆Na₂O₉S₂
• Numer CAS: 1064-48-8
• Masa cząsteczkowa: 616.49
• EC Number: 213-903-1
• UNSPSC Code: 41105322
• NACRES: NA.32
• PubChem Substance ID: 24897642
• MDL number: MFCD00004017
• Beilstein/REAXYS Number: 5374263
• Colour Index Number: 20470

Właściwości

• product line: BioReagent
• Quality Segment: 200
• form: powder
• composition: Dye content, ~85%
• technique(s): protein staining: suitable
• impurities: ≤10% water
• suitability: suitable for electrophoresis
• SMILES string: [Na+].[Na+].Nc1c(\N=N\c2ccc(cc2)[N+]([O-])=O)c(cc3cc(c(\N=N\c4ccccc4)c(O)c13)S([O-])(=O)=O)S([O-])(=O)=O
• InChI: 1S/C22H16N6O9S2.2Na/c23-19-18-12(11-17(39(35,36)37)21(22(18)29)27-24-13-4-2-1-3-5-13)10-16(38(32,33)34)20(19)26-25-14-6-8-15(9-7-14)28(30)31;;/h1-11,29H,23H2,(H,32,33,34)(H,35,36,37);;/q;2*+1/p-2/b26-25+,27-24+;;
• InChI key: AOMZHDJXSYHPKS-DROYEMJCSA-L

Opis

Application

Naphthol Blue Black has been used for the staining of western blot membrane for detection of all protein that are transferred to the membrane.[1]

Biochem/physiol Actions

Naphthol blue black dye can be used to stain proteins on polyacrylamide gels, agarose gels and nitrocellulose membranes. After electrophoresis, fixing the proteins in the gel is recommended. The gel can then be stained with 0.1% Naphthol Blue Black in 7% (v/v) acetic acid for at least 2 hr and destained with a soluion of 7% (v/v) acetic acid. Detection sensitivity is approx. 20% that of Coomassie Blue R.

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Informacja o środkach bezpieczeństwa

• pictograms: GHS07
• signalword: Warning
• hcodes: H317
• pcodes: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Hazard Classifications: Skin Sens. 1B
• Klasa składowania: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Gloves