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Merck

M0535

Sigma-Aldrich

Mycoplasma Broth

powder, suitable for microbiology

Synonim(y):

Mycoplasma media

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About This Item

Kod UNSPSC:
41106200
NACRES:
NA.74

Postać

powder

metody

microbiological culture: suitable

rozpuszczalność

H2O: 25.5 g/L (Autoclave at 121°C, 15 psi for 15 minutes. Store prepared medium at 0-5°C.)

Zastosowanie

microbiology

Opis ogólny

Mycoplasma Broth is a basal medium which, after enrichment with yeast extract, horse serum, and antibiotics, will support the growth of Mycoplasma sp. The components contained in this basal medium do not exhibit inhibitory or toxic effects when used to culture mycoplasma. A special mineral supplement has been added to improve the growth and colony characteristics of the organism.

Zastosowanie

Mycoplasma Broth has been used for isolation and cultivation of mycoplasma. It may also be used to grow Porphyromonas gingivalis under anerobic conditions.

Komponenty

Powder contains (g/L): 10.0 bacterial peptone, 10.0 LAB-LEMCO powder, 5.0 sodium chloride, 0.5 mineral supplement.

Uwaga dotycząca przygotowania

Basal Medium:

1. Suspend 25.5 g of powdered medium in 1L of distilled water. Stir until the powder dissolves.

2. Adjust the pH of the solution to 7.8 ± 0.2.

3. Autoclave at 121 °C and 15 psi for 15 minutes to sterilize. Cool to 50 °C and add sterile supplements.

Przechowywanie i stabilność

Store dry powdered medium at room temperature and prepare medium at 2-8 °C.

Inne uwagi

The product is intended for laboratory use only.
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Kod klasy składowania

10 - Combustible liquids

Klasa zagrożenia wodnego (WGK)

WGK 2

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable


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