L3790

Lysozyme from chicken egg white

10 mg/mL

• Synonim(y): Mucopeptide N-acetylmuramoylhydrolase, Muramidase
• Numer CAS: 12650-88-3
• Numer EC enzymu: 3.2.1.17 (BRENDA, IUBMB)
• Numer MDL: MFCD00131557
• Kod UNSPSC: 12352204
• NACRES: NA.56

Właściwości

• pochodzenie biologiczne: chicken egg white
• Postać: liquid
• masa cząsteczkowa: single-chain 14.3 kDa
• stężenie: ≥400000 units/mL; 10 mg/mL
• metody: cell based assay: suitable
• przydatność: suitable for cell lysis
• numer dostępu UniProt: P00698
• Zastosowanie: cell analysis
• Warunki transportu: wet ice
• temp. przechowywania: −20°C
• informacje o genach: chicken ... LYZ(396218)

Opis

Opis ogólny

Lysosyme is an enzyme found in egg white that breaks down the cell walls in bacteria enhancing the efficiency of DNA and protein extraction.

Zastosowanie

Enzyme breaks down the cell walls of bacteria; used to prepare spheroplasts.

Suitable for the purification of both DNA and protein from bacteria.

Działania biochem./fizjol.

Lysozyme hydrolyzes β(1→4) linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan. Gram-negative bacteria are less susceptible due to the presence of an outer membrane and a lower proportion of peptidoglycan. However, these cells may be hydrolyzed in the presence of EDTA that chelates metal ions in the outer bacterial membrane.

The enzyme is active over a broad pH range (6.0 to 9.0). At pH 6.2, maximal activity is observed over a wider range of ionic strengths (0.02 to 0.100 M) than at pH 9.2 (0.01 to 0.06 M).

Przydatność

Tested for suitability with CelLytic^™ B in protein extraction from bacteria.

Definicja jednostki

One unit will lyse 0.4 μg of Micrococcus lysodeikticus per minute by turbidimetric detection at 600 nm when suspended in buffer at pH 8.0 at 37 °C.

Postać fizyczna

Ready to use solution:10 mg/mL lysozyme in 25 mM sodium acetate pH 4.5 with 50% glycerol.

Solution in 50% glycerol, 25 mM Sodium Acetate

Informacje prawne

CelLytic is a trademark of Sigma-Aldrich Co. LLC

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Informacja o środkach bezpieczeństwa

• Piktogramy: GHS08
• Hasło ostrzegawcze: Danger
• Zwroty wskazujące rodzaj zagrożenia: H334
• Zwroty wskazujące środki ostrożności: P261 - P284 - P501
• Klasyfikacja zagrożeń: Resp. Sens. 1
• Kod klasy składowania: 10 - Combustible liquids
• Klasa zagrożenia wodnego (WGK): WGK 2
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• Środki ochrony indywidualnej: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)