L3382

Lectin from Helix pomatia

lyophilized powder, salt, free

• Synonim(y): Helix pomatia agglutinin, HPA
• Kod UNSPSC: 12352202
• NACRES: NA.32

Właściwości

• Formularz: lyophilized powder
• Poziom jakości: 200
• siła działania: <4 μg per mL agglutination activity
• zanieczyszczenia: salt, free
• temp. przechowywania: −20°C

Opis

Opis ogólny

Lectin from Helix pomatia is a hexameric glycoprotein[1] with a molecular mass of 79 kDa.[2] It issecreted by the albumen gland of the roman snail.[2] Helix pomatia agglutinin (HPA) is specific for N-acetylgalactosamine (GalNAc).[1]

Zastosowanie

Lectin from Helix pomatia has been used to conjugate with biotin and acridinium[3] and used in lectin-agglutination tests.[4]

Działania biochem./fizjol.

HPA has anti-A human blood group specificity and has an affinity for terminal N-acetyl-α-D-galactosaminyl residues. Conjugates are prepared from affinity purified lectin.

Lectin from Helix pomatia protects fertilized eggs by coating them with perivitelline fluid.[2] They exhibit antibacterial activities and immunity in snail.[2] Helix pomatia agglutinin (HPA) possess the potential to agglutinate pathogens.[1] HPA has also emerged as a marker of altered Oglycosylation in cancer.[5]

Uwaga dotycząca przygotowania

Affinity purified by a modification of the method of Hammarstrom and Kabat.[6]

Komentarz do analizy

Where reported, agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of human blood group A erythrocytes after 1 hour incubation at 25 °C.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• Środki ochrony indywidualnej: Eyeshields, Gloves, type N95 (US)