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G3153
β-Galactosidase from Escherichia coli
lyophilized powder, ≥500 units/mg protein
Synonim(y):
β-D-Galactoside galactohydrolase, Lactase
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| Do Państwa/SKU | Dostępność | Cena netto |
|---|---|---|
5 mg | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 2040,00 zł |
Informacje o tej pozycji
Numer CAS:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-864-1
MDL number:
Specific activity:
≥500 units/mg protein
Recombinant:
expressed in E. coli
2040,00 zł
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
Pomoc techniczna
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Pozwól nam pomócrecombinant
expressed in E. coli
Quality Segment
form
lyophilized powder
specific activity
≥500 units/mg protein
mol wt
465 kDa
storage temp.
−20°C
General description
Tetramer molecular weight 465 kDa (subunits 116.3 kDa each)
β-galactosidase is a 465 kDa tetrameric protein. It is coded by the LacZ gene.
Application
β-Galactosidase may be used for derivatization, such as an enzyme label for IgG, without prior dialysis or gel filtration.[1]
β-Galactosidase from Escherichia coli has been used to produce biocatalytic Ca-alginate beads on a B-390 encapsulator (BUCHI). It has been used to prepare the source droplet solution and also as the cargo for Pep-1 (KETWWETWWTEWSQPKKKRKV-cysteamide).
Biochem/physiol Actions
β-galactosidase cleaves lactose into its monosaccharide components, glucose and galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of β-galactosidase, in a feedback loop.
β-galactosidase is considered as a reporter enzyme in applications involving gene expression regulation, analysis of protein function/structure and target gene expression.[2]
Packaging
Package size based on protein content
Physical form
Stabilized with phosphate buffer and sucrose
Other Notes
One unit will hydrolyze 1.0 μmole of o-nitrophenyl β-D-galactopyranoside to o-nitrophenol and D-galactose per min at pH 7.3 at 37 °C.
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Ta pozycja | |||
|---|---|---|---|
| specific activity ≥500 units/mg protein | specific activity ≥250 units/mg protein | specific activity ~140 U/mg | specific activity ≥500 units/mg protein (biuret) |
| recombinant expressed in E. coli | recombinant - | recombinant - | recombinant - |
| form lyophilized powder | form lyophilized powder | form powder | form aqueous glycerol suspension |
| mol wt 465 kDa | mol wt 465 kDa | mol wt Mr ~135000 | mol wt 465 kDa |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| Quality Level 200 | Quality Level 200 | Quality Level 100 | Quality Level 200 |
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Klasa składowania
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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