This product is lyophilized from a solution containing phosphate buffer and sucrose and may be reconstituted in water. For testing purposes, this product is dissolved at 5 mg/mL in a cold assay buffer. See below to review the composition of the 50 mM Tris assay buffer:
1.0 ml 1 M Tris stock solution
0.4 ml of 0.5 M magnesium chloride stock solution
0.01 ml Mercapto-ethanol
Add to 15 ml H2O
adjust pH 7.3 with HCl 1 M
QS to 20 ml
Przejdź do
48275
β-Galactosidase from Escherichia coli
suitable for enzyme immunoassay, lyophilized, powder, ~140 U/mg
Synonim(y):
β-D-Galactoside galactohydrolase, Lactase
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| Do Państwa/SKU | Dostępność | Cena netto |
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Informacje o tej pozycji
Numer CAS:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-864-1
MDL number:
Specific activity:
~140 U/mg
Informacje o cenach i dostępności nie są obecnie dostępne.
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powder
Quality Segment
quality
lyophilized
specific activity
~140 U/mg
mol wt
Mr ~135000
contains
phosphate buffer as stabilizer, sucrose as stabilizer
technique(s)
enzyme immunoassay: suitable
shipped in
wet ice
storage temp.
−20°C
General description
Tetramer molecular weight 465 kDa (subunits 116.3 kDa each)
Application
Aqueous solution may be used directly for derivatization without previous dialysis or gel permeation chromatography.
Biochem/physiol Actions
β-galactosidase cleaves lactose into its monosaccharide components, glucose and galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of β-galactosidase, in a feedback loop.
Packaging
Bottomless glass bottle. Contents are inside inserted fused cone.
Other Notes
1 U corresponds to the amount of enzyme which hydrolyzes 1 μmol 2-nitrophenyl-β-D-galactopyranoside (Cat. No. 73660) per minute at pH 7.8 and 37 °C
Used in a highly sensitive enzyme-linked immunoassay of human IgG with 4-methylumbelliferyl-β-D-galactoside (Catalog No. 69590) [1]; Evaluation of β-D-galactosidase (E. coli) and horse radish peroxidase as labels by sandwich enzyme immunoassay technique [2]; Enzyme immunoassay of thyrptropin releasing hormone (TRH) [3]; Preparation of enzyme-antibody conjugates.
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Ta pozycja | |||
|---|---|---|---|
| specific activity ~140 U/mg | specific activity ≥500 units/mg protein (biuret) | specific activity ≥250 units/mg protein | specific activity ≥500 units/mg protein |
| technique(s) enzyme immunoassay: suitable | technique(s) - | technique(s) - | technique(s) - |
| form powder | form aqueous glycerol suspension | form lyophilized powder | form lyophilized powder |
| mol wt Mr ~135000 | mol wt 465 kDa | mol wt 465 kDa | mol wt 465 kDa |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| shipped in wet ice | shipped in - | shipped in wet ice | shipped in - |
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Klasa składowania
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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