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Merck

E3403

EZview™ Red Protein G Affinity Gel

Synonim(y):

EZview Affinity Gel, Protein G Affinity Gel

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Wybierz wielkość

1 ML

2160,00 zł

5 X 1 ML

4980,00 zł

2160,00 zł

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Informacje o tej pozycji

UNSPSC Code:
12352202
NACRES:
NA.56
MDL number:
MFCD03456743
Form:
liquid

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form

liquid

analyte chemical class(es)

proteins (Immunoglobulins of various mammalian species)

technique(s)

affinity chromatography: suitable, immunoprecipitation (IP): suitable

capacity

≥8 mg/mL, gel binding capacity (rabbit IgG)

storage temp.

−20°C

Quality Level

200

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Ta pozycja
P6486E6779E6406
EZview™ Red Protein G Affinity Gel

Millipore

E3403

EZview™ Red Protein G Affinity Gel

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Millipore

P6486

EZview™ Red Protein A Affinity Gel

EZview™ Red Anti-HA Affinity Gel

Millipore

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EZview™ Red Anti-HA Affinity Gel

EZview™ Red Glutathione Affinity Gel

Millipore

E6406

EZview™ Red Glutathione Affinity Gel

technique(s)

affinity chromatography: suitable, immunoprecipitation (IP): suitable

technique(s)

affinity chromatography: suitable, immunoprecipitation (IP): suitable

technique(s)

affinity chromatography: suitable, immunoprecipitation (IP): suitable

technique(s)

immunoprecipitation (IP): suitable, protein purification: suitable

form

liquid

form

liquid

form

-

form

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

analyte chemical class(es)

proteins (Immunoglobulins of various mammalian species)

analyte chemical class(es)

proteins (Immunoglobulins of various mammalian species)

analyte chemical class(es)

proteins (hemagglutinin)

analyte chemical class(es)

proteins (GST)

capacity

≥8 mg/mL, gel binding capacity (rabbit IgG)

capacity

~4 mg binding capacity (rabbit IgG)

capacity

≥0.4 mg/mL, gel binding capacity (HA-tagged fusion protein)

capacity

-

General description

EZ view Red Protein G Affinity Gel is Protein G covalently bonded to 4% Agarose beads. The affinity gel is used to bind the FC portion of the IgG of an antibody to samples, such as cell lysates and tissue, for purification of proteins in preparation of immunoprecipitation assays. Red dye enhances visability for more efficient results.

Application

When performing small-scale affinity capture, such as immunoprecipitation, the affinity matrix is difficult to see in the microcentrifuge tubes. Accidental aspiration of the resin leads to quantitative variability in results. The EZview™ Red Affinity Gels greatly reduce the risk of pellet loss. EZview resins perform as well as conventional non-colored affinity gels, but allow the user to easily differentiate pellet from supernatant. This correlates to more accurate data because less protein is lost.

Features and Benefits

  • Increased visibility - Red color reduces risk of incidental aspiration
  • Improved recovery of target protein by reduced accidental loss
  • Higher reproducibility - More consistent yields

Physical form

1:1 (v/v) suspension in PBS containing 50% glycerol and 15 ppm Kathon
50% slurry in PBS with 50%Glycerol and 0.0015%

Legal Information

EZview is a trademark of Sigma-Aldrich Co. LLC
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Klasa składowania

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certyfikaty analizy (CoA)

Lot/Batch Number
0000493026
0000493026
SLCG1583
SLBQ8240V
SLBN8308V

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The majority of mammalian genes contain one or more alternative polyadenylation sites. Choice of polyadenylation sites was suggested as one of the underlying mechanisms for generating longer/shorter transcript isoforms. Here, we demonstrate that mature mRNA transcripts can undergo additional cleavage and
Min Mo et al.
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Álvaro Quintanal-Villalonga et al.
EBioMedicine, 53, 102683-102683 (2020-03-03)
Fibroblast growth factor receptor (FGFR)1 and FGFR4 have been associated with tumorigenesis in a variety of tumour types. As a therapeutic approach, their inhibition has been attempted in different types of malignancies, including lung cancer, and was initially focused on
Juri Kazakevych et al.
Nucleic acids research, 45(10), 5770-5784 (2017-03-24)
Epigenetic mechanisms, including chromatin structure, chromatin dynamics and histone modifications play an important role for maintenance and differentiation of pluripotent embryonic stem cells. However, little is known about the molecular mechanisms of adult stem cell specification and differentiation. Here, we
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Lung adenocarcinoma accounts for approximately half of lung cancer cases. Twenty to 50% of tumors of this type harbor mutations affecting epidermal growth factor receptor (EGFR) expression or activity, which can be therapeutically targeted. EGFR inhibitors in this context exhibit
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Product Information Sheet - E3403

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