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D9663

Donkey serum

Synonim(y):

Serum

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Gabaryty przesyłkiSKUDostępnośćCena netto
10 mL
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801,00 zł

Informacje o tej pozycji

NACRES:
NA.46
UNSPSC Code:
12352203
Biological source:
donkey
Sterility:
non-sterile
Shipped in:
dry ice

801,00 zł


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biological source

donkey

Quality Segment

sterility

non-sterile

contains

preservative

application(s)

research pathology

shipped in

dry ice

storage temp.

−20°C

General description

Donkey serum is pooled from a normal donor herd. In immunoassays, normal donkey serum can serve as a blocking agent and a control. It is used as a blocking agent in various studies for immunohistochemistry and immunocytochemistry, ranging from 5% to 20%.[1]

Application

Donkey serum has been used:
  • as a component in blocking buffer for immunofluorescence[2]
  • in phosphate buffer saline (PBS) as a blocking agent[2]
  • as a blocking agent in frozen immunocytochemistry and immunohistochemistry[3]
  • to prevent nonspecific binding in parvalbumin immunohistochemistry[4]

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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G9023R9133S3772
biological source

donkey

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goat

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rabbit

biological source

sheep

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

application(s)

research pathology

application(s)

research pathology

application(s)

-

application(s)

-

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

contains

preservative

contains

preservative

contains

preservative

contains

preservative


flash_point_f

Not applicable

flash_point_c

Not applicable

Klasa składowania

10 - Combustible liquids



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Protokoły

TrueGel3D® Hydrogel Plate protocol guides high-throughput culture of human adipose MSCs for screening applications.

Cultivate ReNcell® human neural stem cells in 3D hydrogels for high-throughput screening using the TrueGel3D® HTS Hydrogel Plate with this protocol.

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Numer pozycji handlu globalnego

SKUNUMER GTIN
D9663-500ML04061837896897
D9663-10ML04061838255259

Questions

1–4 of 4 Questions  
  1. Can this product be frozen and thawed without affecting the quality?

    1 answer
    1. For continuous use, this product should be stored at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Please see the link below to review additional information available in the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/293/677/d9663dat-ms.pdf

      Helpful?

  2. where can I find the protocol to reconstitute this donkey serum

    1 answer
    1. A protocol is not associated with this product. A preservative is added to this serum, making it unsuitable for cell culture. However, in blocking applications, it is typically used at a concentration of 5% - 20%. It may be helpful to view the 'Peer Reviewed Papers' and 'Protocols and Articles' section of the product detail page.

      Helpful?

  3. Is heat inactivation required for D9663 in immunofluorescence (IF)? I found information stating that it is not necessary for immunohistochemistry (IHC). If this applies to IF as well, what is the reason behind it?

    1 answer
    1. Donkey serum does not require heat inactivation for immunofluorescence. It is important to note that this is not a sterile product. While the lab may have routinely heat inactivated their serums for immunofluorescence, this is likely for worker safety rather than a specific requirement for performing immunofluorescence.

      Helpful?

  4. Does donkey serum D9663-10ML require heat inactivation prior to immunohistochemical assay?

    1 answer
    1. There is no need to heat inactivate donkey serum for blocking purposes.

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