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Merck

D8899

Sigma-Aldrich

Deoxyribonucleic acid, single stranded from calf thymus

lyophilized powder

Synonim(y):

DNA single stranded

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About This Item

Numer CAS:
Numer WE:
Numer MDL:
Kod UNSPSC:
41106310
eCl@ss:
32160414
NACRES:
NA.52

klasa czystości

for molecular biology

Poziom jakości

Postać

lyophilized powder

masa cząsteczkowa

~50 kb

skład

ssDNA, ≥65%

temp. przechowywania

−20°C

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Opis ogólny

High quality single-stranded template DNA isolated from the thymus of male and female calves.

Zastosowanie

Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations. It was used as a standard for DNA quantification by fluorescent assay and in DNA-binding assay.
Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations.

Cechy i korzyści

• Activated
• No nuclease activity detected after 16 h at 37 °C incubation
• High quality template DNA

Ilość

One mg DNA is approx. 25 A260 units.

Uwaga dotycząca przygotowania

Prepared by a modification of the method of Alberts and Herrick from calf thymus DNA.

Rekonstytucja

Dissolve the DNA gently rocking overnight to a final concentration of 1mg/ml in molecular biology grade water.

Kod klasy składowania

10 - Combustible liquids

Klasa zagrożenia wodnego (WGK)

WGK 3

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Środki ochrony indywidualnej

Eyeshields, Gloves


Certyfikaty analizy (CoA)

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Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

J A Hansen et al.
Journal of animal science, 75(7), 1810-1821 (1997-07-01)
We evaluated the effects of recombinant porcine somatotropin (pST) and the beta-adrenergic agonist salbutamol on plasam endocrine and metabolite profiles and muscle chemistry in three genotypes of growing barrows (n = 96, 139 d old). Treatments were in a 2
H Kamei
Cell biology international, 20(11), 769-775 (1996-11-01)
The anti-desmin monoclonal antibodies (MAbs) DSB389, AC54, and DSB860 recognize intermediate filaments (IFs) and nuclear antigens that appear granular, locate around chromosomes, and are insoluble following 0.5% Triton X-100 and cetyltrimethylammonium bromide (CTAB) extraction. Nuclear antigens of the MAbs were
José Luis García-Giménez et al.
Journal of inorganic biochemistry, 121, 167-178 (2013-02-07)
Ternary copper(II) complexes [Cu(NST)2(phen)] (1) and [Cu(NST)2(NH3)2]·H2O (2) [HNST=N-(4,5-dimethylthiazol-2-yl)naphthalene-1-sulfonamide] were prepared and characterized by physico-chemical techniques. Both 1 and 2 were structurally characterized by X-ray crystallography. The crystal structures show the presence of a distorted square planar CuN4 geometry in
P Jeslin Kanaga Inba et al.
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, 104, 300-309 (2013-01-01)
Salen [2,2'-{propane-1,3-diylbis[nitrilo(E)methylylidene]}bis(6-methoxyphenol)], reduced salen [(2,2'-[propane-1,3-diylbis(iminomethylene))]bis(6-methoxyphenol)] and N-alkylated salen [diethyl-2,2'-(propane-1,3-diylbis((2-hydroxy-3-methoxybenzyl) azanediyl))diacetate] compounds have been synthesized and characterized by IR, (1)H NMR, (13)C NMR and UV-vis. spectroscopy. Molecular geometry of the title compounds in the ground state has been optimized by density
Qiong Guo et al.
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, 106, 155-162 (2013-02-05)
A divanadium(V) complex, [V2O3(o-van-val)2] (o-van-val=Schiff base derived from o-vanillin and L-valine), has been synthesized and structurally characterized. The crystal structure shows that both of the vanadium centers in the complex have a distorted octahedral coordination environment composed of tridentate Schiff

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