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Kluczowe dokumenty
D4764
Deoxyribonucleic acid from calf thymus
Genomic, unsheared
Synonim(y):
double-stranded template DNA, Calf Thymus DNA, DNA from calf thymus, Thymonucleic acid
Zaloguj sięWyświetlanie cen organizacyjnych i kontraktowych
About This Item
Numer CAS:
Numer WE:
Numer MDL:
Kod UNSPSC:
41106310
eCl@ss:
32160414
NACRES:
NA.52
Polecane produkty
klasa czystości
for molecular biology
Poziom jakości
Formularz
lyophilized powder
masa cząsteczkowa
>20 kb
temp. przechowywania
2-8°C
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Opis ogólny
High quality double-stranded template DNA isolated from the thymus of male and female calves.
Specyficzność
42% GC; Tm = 87 °C.
% GC is the percentage of G/C base pairs. Tm is the temperature at the midpoint of the thermal denaturation profile.
% GC is the percentage of G/C base pairs. Tm is the temperature at the midpoint of the thermal denaturation profile.
Zastosowanie
Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations.
Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations. It was used as a standard for DNA quantification by fluorescent assay. It was used as reference to determine the DNA content in cartilage tissue digest samples.
Cechy i korzyści
• No nuclease activity detected after 16 h at 37 °C incubation
• High quality template DNA
• High quality template DNA
Uwaga dotycząca przygotowania
Purified by anion exchange chromatography, dialyzed against a solution of 1 mM sodium chloride, 1 mM EDTA and 1 mM Trizma HCl, pH 7.5 and lyophilized at a concentration of ~2 A260 units per mL. ~20 A260 units per mg DNA.
Rekonstytucja
Dissolve the DNA gently rocking overnight to a final concentration of 1mg/ml in molecular biology grade water.
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Kod klasy składowania
10 - Combustible liquids
Klasa zagrożenia wodnego (WGK)
WGK 3
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Środki ochrony indywidualnej
Eyeshields, Gloves
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