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Merck

C7527

Choline chloride

BioReagent, suitable for cell culture, suitable for insect cell culture, ≥98%

Synonim(y):

(2-Hydroxyethyl)trimethylammonium chloride

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Wybierz wielkość

100 G

312,00 zł

500 G

892,00 zł

1 KG

1500,00 zł

312,00 zł


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Informacje o tej pozycji

Wzór liniowy:
(CH3)3N(Cl)CH2CH2OH
Numer CAS:
Masa cząsteczkowa:
139.62
UNSPSC Code:
12352205
NACRES:
NA.75
PubChem Substance ID:
EC Number:
200-655-4
Beilstein/REAXYS Number:
3563126
MDL number:

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biological source

synthetic (organic)

Quality Level

product line

BioReagent

assay

≥98%

form

crystals

mol wt

Mw 139.62 g/mol

technique(s)

cell culture | insect: suitable, cell culture | mammalian: suitable, cell culture | plant: suitable

color

white

pH

5.0-6.5 (25 °C, 140 g/L)

mp

302-305 °C (dec.) (lit.)

solubility

H2O: 50 mg/mL

SMILES string

[Cl-].C[N+](C)(C)CCO

InChI

1S/C5H14NO.ClH/c1-6(2,3)4-5-7;/h7H,4-5H2,1-3H3;1H/q+1;/p-1

InChI key

SGMZJAMFUVOLNK-UHFFFAOYSA-M

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Ta pozycja
C1879C7017W450001
Choline chloride ≥98%

C1879

Choline chloride

technique(s)

cell culture | insect: suitable, cell culture | plant: suitable, cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

-

technique(s)

-

biological source

synthetic (organic)

biological source

synthetic

biological source

synthetic

biological source

synthetic

assay

≥98%

assay

≥98%

assay

≥99%

assay

-

form

crystals

form

powder or crystals

form

powder

form

powder, crystals or chunks

solubility

H2O: 50 mg/mL

solubility

-

solubility

-

solubility

-

Quality Level

300

Quality Level

200

Quality Level

200

Quality Level

400

General description

Choline chloride is a quaternary ammonium salt and associates with hydrogen bond donors (HBD). It is essential for the vital physiological functions and is used as a nutritional additive. Choline is a source of methyl groups for methyl-transfer reactions.
Choline acetyltransferase substrate

Application

Choline chloride has been used:
  • as a component of choline acetyltransferase (ChAT) buffer and for choline uptake in cultured human neuroblastoma (SK-N-SH) cells
  • for choline standard curve generation for quantifying phospholipase D (PLD) activity
  • as a component of slice solution for the dissection of entorhinal-hippocampal brain slices

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Klasa składowania

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves


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Certyfikaty analizy (CoA)

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Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Apoptosis of bovine granulosa cells: Intracellular pathways and differentiation
Carou MC, et al.
Acta Histochemica, 119(5), 462-470 (2017)
Synthesis and characterization of choline chloride based binary mixtures
Abbas Q and Binder L
ECS Transactions, 33(7), 49-59 (2010)
Determination of high-affinity choline uptake (HACU) and choline acetyltransferase (ChAT) activity in the same population of cultured cells
Ray B, et al.
Brain Research, 1297(11), 160-168 (2009)
Functional calcium imaging in developing cortical networks
Dawitz J, et al.
Journal of Visualized Experiments, 119(56), e3550-e3550 (2011)
Harvie P Portugaliza et al.
Scientific reports, 9(1), 14595-14595 (2019-10-12)
Transmission of malaria parasites from humans to mosquito vectors requires that some asexual parasites differentiate into sexual forms termed gametocytes. The balance between proliferation in the same host and conversion into transmission forms can be altered by the conditions of

Numer pozycji handlu globalnego

SKUNUMER GTIN
C7527-100G04061833512791
C7527-1KG04061833512807
C7527-500G04061833512920

Questions

1–3 of 3 Questions  
  1. Choline chloride will be melted in water for addition to the medium. Can you please tell me the proper storage method, such as -30°C freezing ay -4°C refrigeration?

    1 answer
    1. This product is soluble in water at 50 mg/mL. The storage and stability conditions for this product upon reconstitution have not been determined.

      Helpful?

  2. Hi, is it possible to thaw choline chloride in PBS instead of water and add it to the medium?

    1 answer
    1. This product is soluble in water, tested at 50 mg/mL. Solubility in PBS has not been determined in-house. Literature suggests that choline chloride can be prepared with PBS. For reference, see https://en-cdn.bio-protocol.org/pdf/Bio-protocol807.pdf?rel_link=YmlvLXByb3RvY29sLm9yZy9lbi9icGRldGFpbD9pZD04MDcmdHlwZT0w

      Helpful?

  3. Hi, could you please provide the product information sheet for Choline chloride.

    1 answer
    1. There is no specific product information sheet offered for Choline Chloride. Please see the PROPERTIES section of the product page for details. Links to a sample Certificate of Analysis and a Certificate of Origin are provided below:

      Certificate of Analysis
      https://www.sigmaaldrich.com/certificates/COFA/C7/C7527/C7527-100G________SLBB8921V_.pdf

      Certificate of Origin
      https://www.sigmaaldrich.com/US/en/coo/undefined/C7527/SLBB8921V

      Helpful?

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