C2273

Carbonic Anhydrase from bovine erythrocytes

For use as a marker in SDS-PAGE

• Synonim(y): Carbonate Dehydratase, Carbonate Hydrolyase
• Numer CAS: 9001-03-0
• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 232-576-6
• MDL number: MFCD00081467
• Numer WE: 4.2.1.1 (BRENDA, IUBMB)
• Biological source: bovine erythrocytes

Właściwości

• biological source: bovine erythrocytes
• Quality Segment: 200
• form: powder
• mol wt: ~29 kDa
• packaging: vial of 5 mg
• storage temp.: 2-8°C

Opis

General description

Carbonic Anhydrase is a metalloenzyme with zinc.[1] The active site of carbonic anhydrase contains three conserved histidine residues (His94, His96 and His119).[2]

Application

Carbonic Anhydrase has been used:

• as a component of nine-protein mixture for the comparative proteomic mass spectrometry analysis of bovine brain samples[3]
• as a molecular marker in gel filtration chromatography[4]
• as a standard protein to test the recovery of CMA/20 microdialysis probe[5]

Biochem/physiol Actions

Carbonic anhydrase is essential for the reversible hydration of carbon dioxide.[1] It is also crucial for the physiological processes including respiration, ion transport, acid–base balance, lipid and carbohydrate metabolic pathways.[1] Carbonic anhydrase participates in electrolyte secretion and in the carbon dioxide fixation.[2]

Other Notes

One collagen digestion unit (CDU) liberates peptides from collagen from bovine achilles tendon equivalent in ninhydrin color to 1.0 μmole of leucine in 5 hours at pH 7.4 at 37 °C.

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Informacja o środkach bezpieczeństwa

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Klasa składowania: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)