C1235

Cholesterol Oxidase microbial

Name: Cholesterol Oxidase microbial
Brand: SIGMA
Price: 1070 PLN

recombinant, lyophilized powder, ≥10 units/mg protein

Synonim(y):

Cholesterol: oxygen oxidoreductase

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100 UNITS

1070,00 zł

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Informacje o tej pozycji

Numer CAS:

9028-76-6

NACRES:

NA.54

UNSPSC Code:

12352204

EC Number:

232-842-1

MDL number:

MFCD00130783

Numer WE:

1.1.3.6 (BRENDA, IUBMB)

Specific activity:

≥10 units/mg protein

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form

lyophilized powder

specific activity

≥10 units/mg protein

mol wt

55 kDa

solubility

50 mM potassium phosphate buffer, pH 7.0: soluble

storage temp.

−20°C

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Ta pozycja	C8868	C5421	C8649
C1235 Cholesterol Oxidase microbial	C8868 Cholesterol Oxidase from microorganisms	C5421 Cholesterol Oxidase from microorganisms	C8649 Cholesterol Oxidase from Streptomyces sp.
specific activity ≥10 units/mg protein	specific activity ≥50 units/mg protein	specific activity ≥30 units/mg protein (biuret)	specific activity ≥20 units/mg protein
form lyophilized powder	form lyophilized powder	form aqueous solution	form lyophilized powder
solubility 50 mM potassium phosphate buffer, pH 7.0: soluble	solubility -	solubility 50 mM potassium phosphate buffer, pH 7.0: soluble (Cold)	solubility 50 mM potassium phosphate buffer, pH 7.0: soluble (Cold)
storage temp. −20°C	storage temp. −20°C	storage temp. −70°C	storage temp. −20°C
mol wt 55 kDa	mol wt 64 kDa	mol wt 62 kDa	mol wt ~34 kDa
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200

Application

Cholesterol oxidase is used to determine serum cholesterol.[1][2] The enzyme also finds application in the microanalysis of steroids in food samples and in distinguishing 3-ketosteroids from 3b-hydroxysteroids.[3] Transgenic plants expressing cholesterol oxidase are being investigated in the fight against the cotton boll weevil.[4] CHOD has also been used as a molecular probe to elucidate cellular membrane structures.[5][6]

Biochem/physiol Actions

Cholesterol oxidase (CHOD) is a monomeric flavoprotein containing FAD that catalyzes the first step in cholesterol catabolism. This bifunctional enzyme oxidizes cholesterol to cholest-5-en-3-one in an FAD-requiring step. This is subsequently isomerized to cholest-4-en-3-one with the release of H₂O₂. Optimum pH of the enzyme is 7.0. Hg²⁺, Ag⁺, ionic detergents inhibit the enzyme activity.

Preparation Note

Dissolves in cold 50 mM potassium phosphate buffer, pH 7.0. Solution is to be prepared just before use.

Other Notes

One unit will convert 1.0 μmol of cholesterol to 4-cholesten-3-one per minute at 37 °C and pH 7.0 in a peroxidase linked system.

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pictograms

GHS08

signalword

Danger

hcodes

H334

pcodes

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Klasa składowania

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Cholesterol-rich fluid membranes solubilize ceramide domains: implications for the structure and dynamics of mammalian intracellular and plasma membranes.

Bruno M Castro et al.

The Journal of biological chemistry, 284(34), 22978-22987 (2009-06-13)

A uniquely sensitive method for ceramide domain detection allowed us to study in detail cholesterol-ceramide interactions in lipid bilayers with low (physiological) ceramide concentrations, ranging from low or no cholesterol (a situation similar to intracellular membranes, such as endoplasmic reticulum)

Electro-chemiluminescent biosensing.

Christophe A Marquette et al.

Analytical and bioanalytical chemistry, 390(1), 155-168 (2007-10-03)

The present review draws a general picture of the bioanalytical applications of electro-chemiluminescent reactions (ECL). Only the two main ECL reactions-i.e. the luminol-based and Ru(bpy)(3)(2+)-based reactions-are considered for application in the fields of enzyme biosensors, immunochemical biosensors, DNA biosensors, and

Performance of four sources of cholesterol oxidase for serum cholesterol determination by the enzymatic endpoint method.

Porntip H Lolekha et al.

Clinica chimica acta; international journal of clinical chemistry, 339(1-2), 135-145 (2003-12-23)

Cholesterol oxidase is used for the determination of serum cholesterol. It can be derived from Streptomyces, Pseudomonas fluorescens, Cellulomonas, and Brevibacterium. This study compared the performance characteristics of four enzymes in the endpoint cholesterol determination. Using the Mega analyzer, we

Cholesterol oxidase senses subtle changes in lipid bilayer structure.

Kwang-wook Ahn et al.

Biochemistry, 43(3), 827-836 (2004-01-21)

We investigated the dependence of cholesterol oxidase catalytic activity and membrane affinity on lipid structure in model membrane bilayers. The binding affinities of cholesterol oxidase to 100-nm unilamellar vesicles composed of mixtures of DOPC or DPPC and cholesterol are not

Purification and characterization of the enzyme cholesterol oxidase from a new isolate of Streptomyces sp.

Vandana Praveen et al.

Applied biochemistry and biotechnology, 165(5-6), 1414-1426 (2011-09-13)

An extracellular cholesterol oxidase (cho) enzyme was isolated from the Streptomyces parvus, a new source and purified 18-fold by ion exchange and gel filtration chromatography. Specific activity of the purified enzyme was found to be 20 U/mg with a 55

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Protokoły

Enzymatic Assay of Cholesterol Oxidase

Enzymatic activity assay for cholesterol oxidase, excluding specific product numbers, providing guidelines for accurate cholesterol oxidase assays.

Powiązane treści

Enzymatic Assay of CHOLESTEROL OXIDASE

Numer pozycji handlu globalnego

SKU	NUMER GTIN
C1235-100UN	04061833467190

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