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C5421
Cholesterol Oxidase from microorganisms
aqueous solution, ≥30 units/mg protein (biuret)
Synonim(y):
Cholesterol:oxygen oxidoreductase
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| Gabaryty przesyłki | SKU | Dostępność | Cena netto |
|---|---|---|---|
| 100 units | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 1030,00 zł | |
| 500 units | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 4050,00 zł |
Informacje o tej pozycji
Numer CAS:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-842-1
MDL number:
Specific activity:
≥30 units/mg protein (biuret)
1030,00 zł
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
Pomoc techniczna
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Pozwól nam pomócform
aqueous solution
Quality Level
specific activity
≥30 units/mg protein (biuret)
mol wt
62 kDa
solubility
50 mM potassium phosphate buffer, pH 7.0: soluble (Cold)
shipped in
dry ice
storage temp.
−70°C
Application
Cholesterol oxidase has been used in a study to improve treatment of oxysterol-mediated cytotoxicity. Cholesterol oxidase has also been used in a study that determined that cholesterol depletion impairs coupling between channel opening and vesicle release by allowing voltage-gated calcium channels to move further from release sites.
Cholesterol oxidase has been used in a study to improve treatment of oxysterol-mediated cytotoxicity. Cholesterol oxidase has also been used in a study that evaluated the effects of cholesterol depletion. This study reported that cholesterol depletion impairs the coupling between channel opening and vesicle release. Additionally, cholesterol oxidase is used to determine serum cholesterol levels.[1][2] The enzyme also finds application in the microanalysis of steroids in food samples. It has also been used for distinguishing 3-ketosteroids from 3β-hydroxysteroids.[3] Transgenic plants expressing cholesterol oxidase have been investigated in the fight against the cotton boll weevil.[4] CHOD has also been used as a molecular probe to elucidate cellular membrane structures.[5][6]
Biochem/physiol Actions
Cholesterol oxidase (CHOD) is a monomeric flavoprotein containing FAD that catalyzes the first step in cholesterol catabolism. This bifunctional enzyme oxidizes cholesterol to cholest-5-en-3-one in an FAD-requiring step. The pH optimum of the enzyme is 7.0 to 7.5 and temperature optimum is 50 °C. The pH stability is 5.7-7.8. Hg2+, Ag+, and ionic detergents inhibit the enzyme activity. Molecular mass of the enzyme is 62 kDa. Pathogenic bacteria require CHOD to infect a host′s macrophage.[7]
Preparation Note
CHOD is soluble in cold 50 mM potassium phosphate buffer, pH 7.0. Prepare solutions immediately before use.
Other Notes
One unit will convert 1.0 μmole of cholesterol to 4-cholesten-3-one per min at pH 7.5 at 25 °C. Note: 4-cholesten-3-one may undergo isomerization.
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Ta pozycja | |||
|---|---|---|---|
| specific activity ≥30 units/mg protein (biuret) | specific activity ≥10 units/mg protein | specific activity ≥20 units/mg protein | specific activity ≥50 units/mg protein |
| form aqueous solution | form lyophilized powder | form lyophilized powder | form lyophilized powder |
| mol wt 62 kDa | mol wt 55 kDa | mol wt ~34 kDa | mol wt 64 kDa |
| shipped in dry ice | shipped in - | shipped in dry ice | shipped in - |
| solubility 50 mM potassium phosphate buffer, pH 7.0: soluble (Cold) | solubility 50 mM potassium phosphate buffer, pH 7.0: soluble | solubility 50 mM potassium phosphate buffer, pH 7.0: soluble (Cold) | solubility - |
| storage temp. −70°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
hcodes
signalword
Danger
pcodes
Hazard Classifications
Resp. Sens. 1
Klasa składowania
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Powiązane treści
QC Methods
Ruchi Chaube et al.
Biochimica et biophysica acta, 1821(2), 313-323 (2011-11-09)
Chronic exposure of blood vessels to cardiovascular risk factors such as free fatty acids, LDL-cholesterol, homocysteine and hyperglycemia can give rise to endothelial dysfunction, partially due to decreased synthesis and bioavailability of nitric oxide (NO). Many of these same risk
Laura Caldinelli et al.
The Journal of biological chemistry, 280(24), 22572-22581 (2005-04-09)
Cholesterol oxidase from Brevibacterium sterolicum is a monomeric flavoenzyme catalyzing the oxidation and isomerization of cholesterol to cholest-4-en-3-one. This protein is a class II cholesterol oxidases, with the FAD cofactor covalently linked to the enzyme through the His(69) residue. In
Porntip H Lolekha et al.
Clinica chimica acta; international journal of clinical chemistry, 339(1-2), 135-145 (2003-12-23)
Cholesterol oxidase is used for the determination of serum cholesterol. It can be derived from Streptomyces, Pseudomonas fluorescens, Cellulomonas, and Brevibacterium. This study compared the performance characteristics of four enzymes in the endpoint cholesterol determination. Using the Mega analyzer, we
Numer pozycji handlu globalnego
| SKU | NUMER GTIN |
|---|---|
| C5421-100UN | 04061832729183 |
| C5421-500UN | 04061832729190 |