C1059

Cloning cylinders, glass

Name: Cloning cylinders, glass
Brand: SIGMA
Price: 399 PLN

volume 150 μL

Synonim(y):

cloning cylinder, cloning equipment

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C1059-1EA

399,00 zł

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Informacje o tej pozycji

NACRES:

NB.22

UNSPSC Code:

41106618

Material:

light brown (tinted)

Sterility:

sterile; electron beam irradiated

Manufacturer/tradename:

Millipore TR1004

Packaging:

dish of 15 cylinders

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material

light brown (tinted)

sterility

sterile; electron beam irradiated

packaging

dish of 15 cylinders

manufacturer/tradename

Millipore TR1004

size

8 mm × 8 mm

volume

150 μL

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Ta pozycja	C3858	C7983	C3983
C1059 Cloning cylinders, glass	Sigma-Aldrich C3858 Scienceware^® cloning cylinders, polystyrene	Sigma-Aldrich C7983 Scienceware^® cloning cylinders, polystyrene	Sigma-Aldrich C3983 Scienceware^® cloning cylinders, polystyrene
sterility sterile; electron beam irradiated	sterility sterile	sterility sterile	sterility sterile
material light brown (tinted)	material -	material -	material -
manufacturer/tradename Millipore TR1004	manufacturer/tradename Bel-Art F37847-0300	manufacturer/tradename Bel-Art F37847-0100	manufacturer/tradename Bel-Art F37847-0200
packaging dish of 15 cylinders	packaging pack of 50 ea	packaging pack of 50 ea	packaging pack of 50 ea
size 8 mm × 8 mm	size -	size -	size -
volume 150 μL	volume -	volume -	volume -

General description

Individual colonies of transfected cells can be isolated and picked from a plate containing many clones. Isolated clones can be dissociated and passaged free from surrounding cells or pulsed with 50-100 uL growth medium which can be analyzed for secreted products. The sterile cloning cylinders are greased on one end to allow the cylinder to seal to the plate. Cells are then dissociated within the cylinder, resuspeneded, and transferred to a new vessel as a pure colony.
These cloning cylinders are sterlized using electron-beam irradiation. The glass cylinders will display a brown tint after being sterlized at the appropriate dosage level.

Packaging

Packaged in 60 mm dish with silicone grease on one end

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Isolation and characterization of trophoblasts from enzymatic explants of human term placenta.

Tamara D Kolokoltsova et al.

Human cell, 30(4), 249-257 (2017-06-15)

In the present study, we describe a new method of isolation and culture of human villous and extravillous trophoblasts from term placenta. The cultivation of trypsinized placental villous tissue explants, followed by the isolation of cells from outgrowth islets allows

Binding of integrin alpha6beta4 to plectin prevents plectin association with F-actin but does not interfere with intermediate filament binding.

D Geerts et al.

The Journal of cell biology, 147(2), 417-434 (1999-10-20)

Hemidesmosomes are stable adhesion complexes in basal epithelial cells that provide a link between the intermediate filament network and the extracellular matrix. We have investigated the recruitment of plectin into hemidesmosomes by the alpha6beta4 integrin and have shown that the

Chromatin and Cytoskeletal Tethering Determine Nuclear Morphology in Progerin-Expressing Cells.

Maria Chiara Lionetti et al.

Biophysical journal, 118(9), 2319-2332 (2020-04-23)

The nuclear morphology of eukaryotic cells is determined by the interplay between the lamina forming the nuclear skeleton, the chromatin inside the nucleus, and the coupling with the cytoskeleton. Nuclear alterations are often associated with pathological conditions as in Hutchinson-Gilford

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