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B0300

Sigma-Aldrich

Betaine solution

5 M, PCR Reagent

Synonim(y):

TMG, trimethylglycine

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About This Item

Wzór liniowy:
(CH3)3N+CH2COO−
Numer CAS:
107-43-7
Masa cząsteczkowa:
117.15
Beilstein:
3537113
Numer MDL:
MFCD00012123
Kod UNSPSC:
12352200
Identyfikator substancji w PubChem:
24891511
NACRES:
NA.52

klasa czystości

PCR Reagent

Poziom jakości

300

Postać

liquid

opakowanie

vial of 1.5 mL

stężenie

5 M

metody

PCR: suitable

kolor

colorless

Zastosowanie

agriculture

obecność zanieczyszczeń

DNase, RNase, none detected

temp. przechowywania

2-8°C

ciąg SMILES

C[N+](C)(C)CC([O-])=O

InChI

1S/C5H11NO2/c1-6(2,3)4-5(7)8/h4H2,1-3H3

Klucz InChI

KWIUHFFTVRNATP-UHFFFAOYSA-N

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Opis ogólny

Betaine also called trimethylglycine or N,N,N triethylammonium acetate, is an analog of glycine with three methyl groups. It is highly compatible with polymerase chain reaction (PCR) buffer mixture. Betaine is a PCR enhancing reagent that is widely used for improving the yield and specificity of PCR products, especially during the PCR amplification of targets rich in GC content or those that form secondary structures resulting in poor yield. Betaine facilitates DNA strand separation and manages the DNA melting temperature (Tm) difference between the GC and AT pairs in DNA. It stabilizes the ds DNA by equalizing the contribution of GC- and AT-base pairs. Betaine has been broadly used to optimize multiplex and ‘long and accurate′ polymerase chain reaction (LA-PCR). The addition of 1.0-1.7 M aqueous betaine to a PCR mixture has been reported to reduce the base pair composition dependence on DNA strand melting.

Zastosowanie

Betaine solution has been used as a component of the reaction mixture for:
  • loop-mediated isothermal amplification (LAMP) reactions
  • polymerase chain reaction for genomic DNA amplification
  • quantitative polymerase chain reaction (qPCR) for telomere length analysis
  • in the PCR amplification of CGG repeats in the genomic DNA
  • reverse-transcription loop-mediated isothermal amplification (RT-LAMP) to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
  • PCR amplification of DNA-directed RNA polymerase II largest subunit (RPB2)
  • reverse transcription during library preparation using Smartseq2

Opakowanie

1.5mL per vial
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Kod klasy składowania

12 - Non Combustible Liquids

Klasa zagrożenia wodnego (WGK)

WGK 1

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

P N Hengen
Trends in biochemical sciences, 22(6), 225-226 (1997-06-01)
Methods and reagents is a unique monthly column that highlights current discussions in the newsgroup bionet.molbio.methds-reagnts, available on the internet. This month's column discusses the use of additives for optimizing the amount and quality of product obtained through multiplex and
Betaine chemistry, roles, and potential use in liver disease
Day CR and Kempson SA
Biochimica et biophysica acta. General subjects, 1860(6), 1098-1106 (2016)
Thanyaornwanya Charoenwijitkul et al.
Clinical biochemistry, 71, 31-37 (2019-06-15)
The 3.7 kb deletion (-α3.7) is the most common form of α+-thalassemia found in multiple populations which can be classified into three subtypes. In order not to mis-identify it, the molecular information within each population is required. We have addressed this
Eniko Sajti et al.
Nature immunology, 21(2), 221-231 (2020-01-22)
The lung is inhabited by resident alveolar and interstitial macrophages as well as monocytic cells that survey lung tissues. Each cell type plays distinct functional roles under homeostatic and inflammatory conditions, but mechanisms establishing their molecular identities and functional potential
Hela Azaiez et al.
Human mutation, 24(4), 305-311 (2004-09-15)
Genetic testing was completed on 1,294 persons with deafness referred to the Molecular Otolaryngology Research Laboratories to establish a diagnosis of DFNB1. Exon 2 of GJB2 was screened for coding sequence allele variants by denaturing high-performance liquid chromatography (DHPLC) complemented
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