A7510

Adenosine 5′-Triphosphatase from porcine cerebral cortex

Name: Adenosine 5′-Triphosphatase from porcine cerebral cortex
Brand: SIGMA

lyophilized powder, ≥0.3 units/mg protein, pH 7.8

Synonim(y):

ATP phosphohydrolase, ATPase

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Numer CAS:

9000-83-3

UNSPSC Code:

12352204

NACRES:

NA.54

Numer WE:

3.6.1.3 (BRENDA, IUBMB)

MDL number:

MFCD00135671

Specific activity:

≥0.3 units/mg protein, pH 7.8

Biological source:

Porcine cerebral cortex

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biological source

Porcine cerebral cortex

description

Sodium and Potassium-Activated

form

lyophilized powder

specific activity

≥0.3 units/mg protein, pH 7.8

composition

Protein, 5.0-25.0% biuret

technique(s)

inhibition assay: suitable

UniProt accession no.

P05024, P05027

storage temp.

−20°C

Quality Level

200

Gene Information

pig ... ATP1A1(397481), ATP1B1(396898)

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Ta pozycja	P6887	SAB5700279	A276
Sigma-Aldrich A7510 Adenosine 5′-Triphosphatase from porcine cerebral cortex	Sigma-Aldrich P6887 Pepsin from porcine gastric mucosa	Sigma-Aldrich SAB5700279 Anti-Phospho-ATM-S1981 antibody produced in rabbit	Sigma-Aldrich A276 Monoclonal Anti-Na⁺/K⁺ ATPase (α Subunit) antibody produced in mouse
technique(s) inhibition assay: suitable	technique(s) -	technique(s) immunofluorescence: 1:50-1:200, western blot: 1:500-1:2000	technique(s) immunocytochemistry: suitable, immunofluorescence: 1:20, immunohistochemistry (frozen sections): 1:100, immunoprecipitation (IP): suitable, indirect ELISA: suitable, western blot: 1:500-1:5000
biological source Porcine cerebral cortex	biological source Porcine gastric mucosa	biological source rabbit	biological source mouse
Gene Information pig ... ATP1A1(397481), ATP1B1(396898)	Gene Information pig ... LOC396892(396892)	Gene Information human ... ATM(472)	Gene Information human ... ATP1A1(476)
specific activity ≥0.3 units/mg protein, pH 7.8	specific activity ≥3,200 units/mg protein	specific activity -	specific activity -
form lyophilized powder	form lyophilized powder	form buffered aqueous solution	form -
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C

Application

ATPase is used to liberate inorganic phosphorus from ATP. ATPase, from porcine cerebral cortex, has been used in inhibition assays of Na/K-ATPase activity [1].

Biochem/physiol Actions

This membrane enzyme hydrolyzes ATP to ADP and orthophosphate. The hydrolysis is coupled with the exchange of sodium and potassium ions across the plasma membrane. The enzyme is present in almost all tissues of higher organisms, but it is most abundant in the kidney where it reabsorbs sodium ions from the glomerular filtrate. Na+,K+-ATPase is affected by changes in intracellular calcium. It is also sensitive to ouabain and inhibited by vanadate, and (in vitro) by SPAI-1, a peptide isolated from porcine duodenum. It has been reported to be regulated by phosphorylation by protein kinase A (PKA). Bufalin, and other cardiotonic steroids, may inhibit Na+/K+-ATPase activity and induce the differentiation of HL60, U937, and ML1 cells [1].

Physical form

Lyophilized powder containing 90% sucrose, 0.4% EDTA Na₄ and 0.06% NaCl

Preparation Note

Sodium iodide extracted fraction of Nakao, et al., Biochem. Biophys. Res. Commun., 19, 755 (1965).

Other Notes

One unit will liberate 1.0 μmole of inorganic phosphorus from ATP per min at pH 7.8 at 37 °C in the presence of Na⁺, K^+, and Mg²⁺.

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related product

Numer produktu

Opis

Cennik

O3125

Ouabain octahydrate, ≥95% (HPLC), powder

Klasa składowania

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Induction by bufalin of differentiation of human leukemia cells HL60, U937, and ML1 toward macrophage/monocyte-like cells and its potent synergistic effect on the differentiation of human leukemia cells in combination with other inducers.

L Zhang et al.

Cancer research, 52(17), 4634-4641 (1992-09-01)

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