A2074
Monoclonal Anti-Rabbit Immunoglobulins–Peroxidase antibody produced in mouse
clone RG-16, purified immunoglobulin, lyophilized powder
Synonim(y):
Monoclonal Anti-Rabbit Immunoglobulins (IgG, IgA, IgM)
Zaloguj sięWyświetlanie cen organizacyjnych i kontraktowych
About This Item
Kod UNSPSC:
12352203
NACRES:
NA.46
Polecane produkty
pochodzenie biologiczne
mouse
Poziom jakości
białko sprzężone
peroxidase conjugate
forma przeciwciała
purified immunoglobulin
rodzaj przeciwciała
secondary antibodies
klon
RG-16, monoclonal
Postać
lyophilized powder
reaktywność gatunkowa
rabbit
spodziewany brak reakcji z
pig, chicken, bovine, horse, human, guinea pig, turkey, rat, canine, goat, feline, sheep
opakowanie
vial of 0.5 mL
metody
direct ELISA: 1:30,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100 using human tissue
western blot (chemiluminescent): 1:160,000
izotyp
IgG1
temp. przechowywania
2-8°C
docelowa modyfikacja potranslacyjna
unmodified
Szukasz podobnych produktów? Odwiedź Przewodnik dotyczący porównywania produktów
Powiązane kategorie
Opis ogólny
Monoclonal Anti-Rabbit Immunoglobulins (mouse IgG1 isotype) is derived from the RG-16 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with rabbit IgG. Immunoglobulins (Igs) belongs to the immunoglobulin super-family. There are five different types of Immunoglobulins. Each immunoglobulin has two heavy (H) and two light (L) chains held together by disulfide linkages. Each light comprises one variable N-terminal region and a constant C-terminal region. Heavy chain has one variable N-terminal region and three or four constant (CH1-CH4) C-terminal region. IgG is abundant immunoglobulin in human serum. The four classes of IgG can be IgG1, IgG2, IgG3, and IgG4.
Reacts with an epitope on the heavy chain of rabbit IgG, IgA and IgM, which is sensitive to reduction. No cross-reactivity with human IgG, IgA and IgM.
Specyficzność
Binds only rabbit Igs. Does not react with reduced rabbit Igs.
Zastosowanie
Monoclonal Anti-Rabbit Immunoglobulins-Peroxidase antibody produced in mouse has been used in:
- western blotting
- immunoblotting
- immunoprecipitation
- enzyme linked immunosorbent assay (ELISA)
- immunohistochemistry
Mouse monoclonal anti-rabbit immunoglobulins have been used for western blot assays at a dilution of 1:1000 using bovine trabecular meshwork cell lysates. The antibody can also be used for immunohistochemistry assays (1:100, using human tissue).
The mouse monoclonal anti-Rabbit IgG (heavy chain-specific) peroxidase conjugated antibody can be used as a secondary antibody for western blot analysis of protein preps from E. coli cultures at a concentration of 1:10000 diluted in PBSt for 1 hour at room temperature.
Postać fizyczna
Lyophilized from 0.01 M sodium phosphate buffer, pH 7.4, containing 1% bovine serum albumin and 0.05% MIT
Uwaga dotycząca przygotowania
Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
This page may contain text that has been machine translated.
Not finding the right product?
Try our Narzędzie selektora produktów.
Hasło ostrzegawcze
Warning
Zwroty wskazujące rodzaj zagrożenia
Zwroty wskazujące środki ostrożności
Klasyfikacja zagrożeń
Skin Sens. 1
Kod klasy składowania
11 - Combustible Solids
Klasa zagrożenia wodnego (WGK)
WGK 2
Środki ochrony indywidualnej
Eyeshields, Gloves, type N95 (US)
Certyfikaty analizy (CoA)
Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.
Masz już ten produkt?
Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
Cheng Chen et al.
European journal of histochemistry : EJH, 65(3) (2021-10-01)
Individuals with diabetes are exposed to a higher risk of perioperative stroke than non-diabetics mainly due to persistent hyperglycemia. LncRNA Meg3 has been considered as an important mediator in regulating ischemic stroke. However, the functional and regulatory roles of Meg3
Nazish Ahmed et al.
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 20(5), 665-678 (2007-09-01)
Chondral defects show lack of proper regeneration whereas osteochondral lesions display limited regeneration capacity. Latter is probably due to immigration of chondroprogenitor cells from the subchondral bone. Known chondroprogenitor cells for cartilage tissues are multi-potent adult marrow stromal or mesenchymal
Pei-Hua Li et al.
Oncology letters, 15(4), 5137-5142 (2018-03-20)
c-Jun activation domain-binding protein-1 (Jab1) is a multifunctional protein involved in cell proliferation and apoptosis, DNA damage and repair and genome stability. In a number of types of human carcinoma, the abnormal expression of Jab1 is associated with poor prognosis
Martina Redova et al.
International journal of molecular medicine, 25(2), 271-280 (2010-01-01)
We investigated the possible modulation by LOX/ COX inhibitors of all-trans retinoic acid (ATRA)-induced cell differentiation in two established neuroblastoma cell lines, SH-SY5Y and SK-N-BE(2). Caffeic acid, as an inhibitor of 5-lipoxygenase, and celecoxib, as an inhibitor of cyclooxygenase-2, were
Isabelle Gillot et al.
International journal of cell biology, 2009, 524858-524858 (2010-02-11)
During prophase I of male meiosis, the sex chromosomes form a compact structure called XY body that associates with the nuclear membrane of pachytene spermatocytes. Ret Finger Protein is a transcriptional repressor, able to interact with both nuclear matrix-associated proteins
Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.
Skontaktuj się z zespołem ds. pomocy technicznej