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53366
Anti-Mouse IgG−Abberior® STAR 488 antibody produced in goat
for STED application
Synonim(y):
Abberior® STAR 488-Anti--Mouse−IgG antibody produced in goat
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About This Item
Kod UNSPSC:
12352200
NACRES:
NA.32
pochodzenie biologiczne
goat
Poziom jakości
forma przeciwciała
affinity isolated antibody
rodzaj przeciwciała
secondary antibodies
klon
polyclonal
Postać
buffered aqueous solution
reaktywność gatunkowa
mouse
dostępność
available only in USA and Canada
stężenie
~1 mg/mL
fluorescencja
λex 503 nm; λem 524 nm in PBS, pH 7.4
temp. przechowywania
−20°C
Opis ogólny
Abberior STAR 488 was developed for STED and confocal microscopy in the green spectral region. It is a bright green fluorescent dye. The dye works extremely well with Abberior Instruments Expert Line microscopes and Leica STED microscopes. It can be very effectively excited with the prominent 488 nm laser line. For STED microscopy, Abberior STAR 488 can be efficiently used with STED Laser wavelengths between 590 - 610 nm. Abberior STAR 488 can substitute dyes like Oregon Green 488, Atto™ 488 or Alexa Fluor™ 488. Abberior STAR 488 is the ideal partner for Abberior STAR 440SXP to obtain optimal 2 Color STED results. Best results are obtained with freshly prepared samples.
Photophysical properties (carboxylic acid):
Absorption Maximum, λex [nm]: 503 (PBS pH 7.4), 504 (H2O), 507 (MeOH + 0.1% TFA)
Extinction Coefficient, εmax [M-1cm-1]: 65 000 (PBS pH 7.4), 80 000 (H2O), 85 000 (MeOH + 0.1% TFA)
Correction Factor, CF260 = ε260/εmax: 0,28 (PBS pH 7.4)
Correction Factor, CF280 = ε280/εmax: 0,14 (PBS pH 7.4)
Fluorescence Maximum, λem [nm]: 524 (PBS pH 7.4), 525 (H2O), 531 (MeOH + 0.1% TFA)
Recommended STED Wavelength, λ [nm]: 590 - 610
Fluorescence Quantum Yield, λ: 0,89 (PBS pH 7.4)
Fluorescence Lifetime, τ [ns]: 3,9 (PBS pH 7.4)
Photophysical properties (carboxylic acid):
Absorption Maximum, λex [nm]: 503 (PBS pH 7.4), 504 (H2O), 507 (MeOH + 0.1% TFA)
Extinction Coefficient, εmax [M-1cm-1]: 65 000 (PBS pH 7.4), 80 000 (H2O), 85 000 (MeOH + 0.1% TFA)
Correction Factor, CF260 = ε260/εmax: 0,28 (PBS pH 7.4)
Correction Factor, CF280 = ε280/εmax: 0,14 (PBS pH 7.4)
Fluorescence Maximum, λem [nm]: 524 (PBS pH 7.4), 525 (H2O), 531 (MeOH + 0.1% TFA)
Recommended STED Wavelength, λ [nm]: 590 - 610
Fluorescence Quantum Yield, λ: 0,89 (PBS pH 7.4)
Fluorescence Lifetime, τ [ns]: 3,9 (PBS pH 7.4)
Przydatność
Designed and tested for fluorescent super-resolution microscopy
Komentarz do analizy
May contain significant amounts of BSA as stabilizer
unconjugated dye ≤5% of total fluorescence
unconjugated dye ≤5% of total fluorescence
Inne uwagi
Informacje prawne
ALEXA FLUOR is a trademark of Life Technologies
Atto is a trademark of Atto-Tec GmbH
abberior is a registered trademark of Abberior GmbH
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Kod klasy składowania
12 - Non Combustible Liquids
Klasa zagrożenia wodnego (WGK)
WGK 3
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
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Beitraege zur Theorie des Mikroskops und der mikroskopischen Wahrnehmung.
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