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Merck

D9779

DL-Dithiothreitol

Molecular Biology, ≥98% (HPLC), ≥99% (titration)

Synonim(y):

(±)-Dithiothreitol, rac-Dithiothreitol, Dithiothreitol, threo-1,4-Dimercapto-2,3-butanediol, Cleland’s reagent, DTT

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250 MG

240,00 zł

1 G

496,00 zł

5 G

1530,00 zł

10 G

2930,00 zł

25 G

5910,00 zł

50 G

9950,00 zł

240,00 zł

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Informacje o tej pozycji

Wzór liniowy:
HSCH2CH(OH)CH(OH)CH2SH
Numer CAS:
3483-12-3
Masa cząsteczkowa:
154.25
UNSPSC Code:
12161504
NACRES:
NA.31
PubChem Substance ID:
24894330
EC Number:
222-468-7
Beilstein/REAXYS Number:
1719757
MDL number:
MFCD00004877

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InChI key

VHJLVAABSRFDPM-QWWZWVQMSA-N

InChI

1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m1/s1

SMILES string

O[C@H](CS)[C@H](O)CS

grade

Molecular Biology

assay

≥98% (HPLC), ≥99% (titration)

form

powder

reaction suitability

reagent type: reductant

mp

41-44 °C (lit.)

solubility

H2O: 50 mg/mL

cation traces

heavy metals (as Pb): ≤5 ppm

suitability

suitable for molecular biology

foreign activity

DNase, RNase, protease, none detected

storage temp.

2-8°C

Quality Level

200

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Ta pozycja
D0632D5545D9163
DL-Dithiothreitol Molecular Biology, ≥98% (HPLC), ≥99% (titration)

Sigma-Aldrich

D9779

DL-Dithiothreitol

DL-Dithiothreitol ≥98% (HPLC), ≥99.0% (titration)

Sigma-Aldrich

D0632

DL-Dithiothreitol

DL-Dithiothreitol BioXtra, ≥99.0% (titration)

Sigma-Aldrich

D5545

DL-Dithiothreitol

DL-Dithiothreitol suitable for electrophoresis, ≥99%

Sigma-Aldrich

D9163

DL-Dithiothreitol

grade

for molecular biology

grade

-

grade

-

grade

-

assay

≥98% (HPLC), ≥99% (titration)

assay

≥98% (HPLC), ≥99.0% (titration)

assay

≥99.0% (titration)

assay

≥99%

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

mp

41-44 °C (lit.)

mp

41-44 °C (lit.)

mp

41-44 °C (lit.)

mp

40-44 °C, 41-44 °C (lit.)

form

powder

form

powder

form

powder

form

powder

solubility

H2O: 50 mg/mL

solubility

H2O: soluble 50 mg/mL, clear, colorless to very faintly yellow

solubility

H2O: 0.1 M, clear, colorless

solubility

H2O: 50 mg/mL, clear, colorless to very faintly yellow

General description

Dithiothreitol (DTT) is an excellent reagent for maintaining SH groups in a reduced state in proteins. It initiates the thiol-disulfide exchange reaction to completely reduce the intra-or inter-molecular disulfide bonds in biomolecules. DTT specifically targets the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel and sodium dodecyl polyacrylamide gel electrophoresis (SDS-PAGE). Hence, it is highly effective in use with buffers that quantitatively reduce disulfides. DTT is less pungent and less toxic in nature than 2-mercaptoethanol. It is also 7x more concentrated than beta-mercaptoethanol.

Application

An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).
DL-Dithiothreitol is used in protein samples to reduce the disulfide bonds before loading the sample onto the gel for Western Blot or SDS-PAGE[1][2] It has been used in the procedure of in situ hybridization of mRNA.[3][3]

It has been used:
  • as a component for protein extraction in western blot analysis[4]
  • to prepare sample lysis buffer for quantitative mass spectroscopy[5]
  • as a kinase buffer component for enzyme-linked immunosorbent assay (ELISA)[6]

Biochem/physiol Actions

Dithiothreitol (DTT) is frequently utilized in biochemical preparations of thiol proteins and chemical peptide synthesis. DTT is also used in protein chemistry research on the folding of proteins and enzyme activity.[7]

Features and Benefits

  • Suitable for molecular biology
  • RNase, DNase, Exonuclease, and Protease-free
  • High purity (HPLC ≥98%)
  • No heavy metal ≤5ppm

Other Notes

For additional information on our range of Biochemicals, please complete this form.
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pictograms

CorrosionExclamation mark
GHS05,GHS07

signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1A

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Klasa składowania

8A - Combustible corrosive hazardous materials

Wybierz jedną z najnowszych wersji:

Certyfikaty analizy (CoA)

Lot/Batch Number
0000539266
0000539265
0000539263
0000539261
0000539270

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Jeśli potrzebujesz konkretnej wersji, możesz wyszukać konkretny certyfikat według numeru partii lub serii.

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Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

A quantitative in situ hybridization protocol for formalin-fixed paraffin-embedded archival post-mortem human brain tissue.
Liu CQ et al.
Methods, 52, 359-359 (2010)
Proteomic Approach for Extracting Cytoplasmic Proteins from Streptococcus sanguinis using Mass Spectrometry
Fadi El-Rami, et al.
Journal of Molecular Biology (2017)
Benjamin Ziman et al.
STAR protocols, 2(1), 100274-100274 (2021-01-26)
In the planarian field, two techniques are mostly used for protein detection: immunohistochemistry (IHC) and western blotting. While IHC is great for visualizing the spatial distribution of proteins in whole organisms, it has limitations in antibody availability and issues related
Dan Su et al.
Evidence-based complementary and alternative medicine : eCAM, 2019, 4982062-4982062 (2019-05-17)
The Janus kinases (JAKs) consist of four similar tyrosine kinases and function as key hubs in the signaling pathways that are implicated in both innate and adaptive immunity. Among the four members, JAK3 is probably the more attractive target for
Tommy Weiss-Sadan et al.
Theranostics, 9(20), 5731-5738 (2019-09-20)
Despite the common use of lipid-lowering medications, cardiovascular diseases continue to be a significant health concern. Atherosclerosis, one of the most frequent causes of cardiovascular morbidity, involves extensive inflammatory activity and remodeling of the vascular endothelium. This relentless inflammatory condition
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Protokoły

Oligo Reduction

Follow this DDT reduction protocol to reduce disulfide bonds in thiol-modified oligonucleotides.

Redukcja oligo

Postępuj zgodnie z tym protokołem redukcji DDT, aby zredukować wiązania dwusiarczkowe w oligonukleotydach modyfikowanych tiolami.

Powiązane treści

mRNA Synthesis

Tools for plasmid preparation, in vitro transcription, mRNA purification, and LNP formulation in mRNA-based vaccine and therapeutic development.

Synteza mRNA

Narzędzia do przygotowywania plazmidów, transkrypcji in vitro, oczyszczania mRNA i formułowania LNP w rozwoju szczepionek i terapii opartych na mRNA.

Questions

1–8 of 8 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. In het kader van de IVDR richtlijnen heb ik de volgende vraag; Heeft de stof DL-Dithiothreitol het keurmerk IVD/CE?

    1 answer

    1. No, this product does not have the have the IVD/CE quality mark. This is a research grade product which is not intended for in vitro applications.

      Helpful?

  3. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  4. What is the shelf life of Product D9779, DL-Dithiothreitol?

    1 answer

    1. The recommended retest period for D9779 is 4 years. This information can be found on the Specification Sheet. The actual retest date for a given lot can be found on the Certificate of Analysis for that lot.

      Helpful?

  5. What is the meaning of Molecular Biology Grade in the description of Product D9779, DL-Dithiothreitol?

    1 answer

    1. Molecular Biology grade products are tested for DNase, RNase, and protease to make certain that they contain minimal levels of these contaminants.

      Helpful?

  6. What is the solubility of Product D9779, DL-Dithiothreitol?

    1 answer

    1. This product is soluble in water (50 mg/mL), yielding a clear, colorless solution. DTT is also soluble in ethanol, acetone, ethyl acetate, chloroform and ether. This information may be found on the product information sheet for a related DTT product (Product No. D0632).

      Helpful?

  7. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  8. What is the solution stability of Product D9779, DL-Dithiothreitol (DTT)?

    1 answer

    1. We generally recommend preparing DTT solutions fresh daily. A table showing the half life of DTT in solution under various conditions can be found on the product information sheet for a related DTT product (Product No. D0632).

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