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S9430

Sigma-Aldrich

SYBR® Green I nucleic acid gel stain

greener alternative

10,000 × in DMSO

Synonim(y):

DNA stain, SYBR® green gel dye, safer gel stain

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About This Item

Numer CAS:
163795-75-3
Numer MDL:
MFCD00284715
Kod UNSPSC:
12171500
NACRES:
NA.52

Postać

solution

Poziom jakości

200

zastosowanie

1.0 mL sufficient for 100 mini-gels

charakterystyka ekologicznej alternatywy

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

stężenie

10,000 × in DMSO

metody

PCR: suitable

kategoria ekologicznej alternatywy

Aligned,

temp. przechowywania

−20°C

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Opis ogólny

SYBR® Green I is a proprietary asymmetrical cyanine dye, which is used to detect nucleic acids. It consists of a N-alkylated benzothiazolium or benzoxazolium ring system, that is joined by a monomethine bridge to a pyridinium or quinolinium ring system. SYBR Green I binds to the minor groove of dsDNA and is excited at a wavelength of 480 nm. It has a peak fluorescence emission of 520 nm.

Zastosowanie

SYBR® Green I nucleic acid gel stain has been used for:
  • the quantification of dsDNA
  • to stain DNA in polymerase chain reaction (PCR)
  • for comet assay technique
  • to assess spermatozoon membrane integrity
  • for visual inspection of DNA amplified by loop-mediated isothermal amplification (LAMP)
  • as a fluorescent dye in flow cytometry
  • real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR) for staining reverse-transcribed cDNA

Cechy i korzyści

  • An ultrasensitive stain for post-electrophoresis staining of dsDNA in agarose or polyacrylamide gels
  • It can also detect ssDNA and RNA in denaturing agarose/formaldehyde and polyacrylamide/urea gels without any pre-washing steps
  • It is less mutagenic than ethidium bromide in Ames tests
  • It provides 50-100 times greater detection sensitivity than ethidium bromide for oligonucleotides
  • Useful for many applications with a limited amount of DNA
  • The binding of SYBR® Green I to DNA does not inhibit the activity of many common restriction endonucleases, including Hind III and EcoR I
  • Removal of this stain in-gel digestion and ligation techniques is not needed
  • SYBR Green I is a greener alternative product to ethidium bromide for staining

Przechowywanie i stabilność

Store the product at –20 °C. The diluted Staining Solution may be stored, protected from light, either at 2–8 °C for several weeks or at room temperature for 3–4 days.

Informacje prawne

SYBR is a registered trademark of Life Technologies
This page may contain text that has been machine translated.

produkt powiązany

Numer produktu
Opis
Cennik

Kod klasy składowania

10 - Combustible liquids

Klasa zagrożenia wodnego (WGK)

WGK 3

Temperatura zapłonu (°F)

201.2 °F - closed cup

Temperatura zapłonu (°C)

94 °C - closed cup

Środki ochrony indywidualnej

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Choose from one of the most recent versions:

Certyfikaty analizy (CoA)

Lot/Batch Number
0000371131
SLCP5377
MKCW7943
MKCW1810
SLCP5377

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If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Shear-resistant hydrogels to control permeability of porous tubular scaffolds in vascular tissue engineering
Tresoldi C, et al.
Materials Science and Engineering, C, 105, 110035-110035 (2019)
Loop mediated isothermal amplification of 5.8S rDNA for specific
detection of Tritrichomonas foetus
Jorge Oyhenart
Veterinary Parasitology, 193 (2013)
I Ortiz et al.
Animal reproduction science, 187, 74-78 (2017-10-19)
The aim of this study was to compare sperm DNA fragmentation of frozen-thawed epididymal sperm of dogs using the SCSA (Sperm Chromatin Structure Assay) and SCDt (Sperm Chromatin Dispersion test). For this purpose, epididymis from neutered dogs were minced and
The in vitro effect of nonylphenol, propranolol, and diethylstilbestrol on quality parameters and oxidative stress in sterlet (Acipenser ruthenus) spermatozoa
Shaliutina O, et al.
Toxicology in vitro, 43, 9-15 (2017)
Deborah Traversi et al.
Scientific reports, 10(1), 17566-17566 (2020-10-18)
Type 1 diabetes (T1D) is a common autoimmune disease that is characterized by insufficient insulin production. The onset of T1D is the result of gene-environment interactions. Sociodemographic and behavioural factors may contribute to T1D, and the gut microbiota is proposed
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