114375

Bromocresol Purple

BioReagent, suitable for indicator, Dye content 90 %

• Synonim(y): 5,5′-Dibromo-o-cresolsulfonphthalein, Bromcresol purple sultone form
• Wzór empiryczny (zapis Hilla): C₂₁H₁₆Br₂O₅S
• Numer CAS: 115-40-2
• Masa cząsteczkowa: 540.22
• UNSPSC Code: 12171500
• NACRES: NA.47
• PubChem Substance ID: 24847168
• EC Number: 204-087-8
• Beilstein/REAXYS Number: 359618
• MDL number: MFCD00011681

Właściwości

• product line: BioReagent
• Quality Level: 200
• assay: ≥89.0% (HPLC)
• form: powder
• composition: Dye content, 90%
• technique(s): titration: suitable
• pH: 5.2-6.8, yellow to purple
• visual transition interval: 5.2-6.8, yellow to blue
• mp: 240 °C (dec.) (lit.)
• solubility: ethanol: 1 mg/mL
• λmax: 419 nm
• ε (extinction coefficient): ≥22000 at 418-424 nm in methanol
• suitability: suitable for indicator
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: Cc1cc(cc(Br)c1O)C2(OS(=O)(=O)c3ccccc23)c4cc(C)c(O)c(Br)c4
• InChI: 1S/C21H16Br2O5S/c1-11-7-13(9-16(22)19(11)24)21(14-8-12(2)20(25)17(23)10-14)15-5-3-4-6-18(15)29(26,27)28-21/h3-10,24-25H,1-2H3
• InChI key: ABIUHPWEYMSGSR-UHFFFAOYSA-N

Opis

Application

Spectrophotometric determination of serum-albumin

Bromocresol Purple has been used for staining of Saccharomyces cerevisiae.

Biochem/physiol Actions

Bromocresol purple is a pH indicator which changes color from yellow (at low pH 5.2) to violet (above pH 6.8). It is mainly used as a fluorescent stain in yeast cells. It helps in dead cell count for cells with plasma membrane damage. Cells with proper cell membrane are not stained, while damaged cells are seen as blue-grey ghosts.

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Informacja o środkach bezpieczeństwa

• Klasa składowania: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Gloves