UC0E03
UCOE® Dual Expression Puromycin Vector Set
Synonim(y):
UCOE Mammalian Protein Expression Vectors, UCOE Mammalian Protein Expression Plasmids, Ubiquitous Chromatin Opening Element, UCOE03
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| Gabaryty przesyłki | SKU | Dostępność | Cena netto |
|---|---|---|---|
| 1 kit | Przewidywana wysyłka DZISIAJzKuehne + Nagel Sp. z o.o. | 6300,00 zł |
Informacje o tej pozycji
UNSPSC Code:
41106621
NACRES:
NA.51
eCl@ss:
34360190
6300,00 zł
Przewidywana wysyłka DZISIAJSzczegóły
Pomoc techniczna
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Pozwól nam pomócshipped in
dry ice
Quality Level
General description
The Ubiquitous Chromatin Opening Element (UCOE) technology offers significant advances in recombinant mammalian protein expression. Unlike traditional vectors, UCOE-containing vectors have the capacity to ease the process of isolating cell line clones that express high-levels of recombinant proteins. UCOE sequences promote reproducible and stable high level expression of a linked gene by altering chromatin structure to a transcriptionally permissive state that negates epigenetic-mediated (e.g. DNA methylation) silencing.
CET 1019 HD-puro-SceI is a mammalian expression plasmid vector containing both the mouse Rps3 UCOE and the human HNRPA2B1-CBX3 UCOE each upstream of a strong human cytomegalovirus (hCMV) immediate early promoter-enhancer element.
CET 1019 AD-puro-SceI is a mammalian expression plasmid vector containing both the mouse Rps3 UCOE and the human HNRPA2B1-CBX3 UCOE each upstream of a guinea pig CMV (gpCMV) promoter-enhancer element.
The CET 1019 HD-puro-SceI and CET 1019 AD-puro-SceI vectors possess the following features:
THIS PRODUCT IS ONLY AVAILABLE FOR SALE TO ACADEMIC INSTITUTIONS OR NOT-FOR-PROFIT ENTITIES FOR USE UNDER THIS PRODUCT LABEL LICENSE. FOR INFORMATION ON COMMERCIAL LICENSING OF THE PATENTED UCOE TECHNOLOGY, PLEASE CONTACT [email protected].
CET 1019 HD-puro-SceI is a mammalian expression plasmid vector containing both the mouse Rps3 UCOE and the human HNRPA2B1-CBX3 UCOE each upstream of a strong human cytomegalovirus (hCMV) immediate early promoter-enhancer element.
CET 1019 AD-puro-SceI is a mammalian expression plasmid vector containing both the mouse Rps3 UCOE and the human HNRPA2B1-CBX3 UCOE each upstream of a guinea pig CMV (gpCMV) promoter-enhancer element.
The CET 1019 HD-puro-SceI and CET 1019 AD-puro-SceI vectors possess the following features:
- A multiple cloning site downstream of the UCOE-CMV combinations containing unique restriction enzyme sites to permit easy insertion of any gene of interest (cDNA or genomic)
- A polyadenylation element from the SV40 early region located downstream of the multiple cloning site for appropriate mRNA 3 end formation
- A puromycin antibiotic resistance gene for selection of stably transfected mammalian cells
- A β-lactamase gene for plasmid selection and propagation in transformed prokaryotic cells in the presence of ampicillin
- An I-SceI homing restriction endonuclease site for linearization of constructs prior to transfection into mammalian cells, which favors integration into the target cell genome via the pre-generated free DNA ends and thus retains the integrity of the UCOE-CMV-transgene cassettes
THIS PRODUCT IS ONLY AVAILABLE FOR SALE TO ACADEMIC INSTITUTIONS OR NOT-FOR-PROFIT ENTITIES FOR USE UNDER THIS PRODUCT LABEL LICENSE. FOR INFORMATION ON COMMERCIAL LICENSING OF THE PATENTED UCOE TECHNOLOGY, PLEASE CONTACT [email protected].
Other Notes
1) 10ug CET 1019 HD-puro-SceI (+) Vector (CS221292)
2) 10ug CET 1019 AD-puro-SceI (+) Vector (CS221293))
3) 10ug DC HD-puro (-) Vector (CS221283)
4) 10ug DC AD-puro (-) Vector (CS221295)
2) 10ug CET 1019 AD-puro-SceI (+) Vector (CS221293))
3) 10ug DC HD-puro (-) Vector (CS221283)
4) 10ug DC AD-puro (-) Vector (CS221295)
Concentration: Please refer to lot specific datasheet.
Legal Information
UCOE is a registered trademark of Merck KGaA, Darmstadt, Germany
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Ta pozycja | |||
|---|---|---|---|
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in dry ice |
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
Klasa składowania
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
Certyfikaty analizy (CoA)
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Masz już ten produkt?
Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
Michael Antoniou et al.
Genomics, 82(3), 269-279 (2003-08-09)
The genetic elements that are responsible for establishing a transcriptionally competent, open chromatin structure at a region of the genome that consists only of ubiquitously expressed, housekeeping genes are currently unknown. We demonstrate for the first time through functional analysis
Fang Zhang et al.
Molecular therapy : the journal of the American Society of Gene Therapy, 18(9), 1640-1649 (2010-07-01)
DNA methylation may restrict the activity of gene transfer vectors due to inadvertent silencing. In P19 embryonic carcinoma cells in vitro, we found that transgene expression regulated by the SFFV LTR and EF1 alpha promoter declined rapidly within 16 days
Numer pozycji handlu globalnego
| SKU | NUMER GTIN |
|---|---|
| UC0E03 | 04054839077616 |