MABS1171
Anti-MPST Antibody, clone 1H10.1
clone 1H10.1, from mouse
Synonim(y):
3-mercaptopyruvate sulfurtransferase, MpST, MST, tRNA thiouridine modification protein, TUM1
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About This Item
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Polecane produkty
pochodzenie biologiczne
mouse
Poziom jakości
forma przeciwciała
purified immunoglobulin
rodzaj przeciwciała
primary antibodies
klon
1H10.1, monoclonal
reaktywność gatunkowa
human
metody
immunohistochemistry: suitable (paraffin)
western blot: suitable
izotyp
IgG2aκ
numer dostępu NCBI
numer dostępu UniProt
Warunki transportu
wet ice
docelowa modyfikacja potranslacyjna
unmodified
informacje o genach
human ... MPST(4357)
Opis ogólny
3-mercaptopyruvate sulfurtransferase (EC 2.8.1.2; UniProt P25325; also known as MpST, MST, tRNA thiouridine modification protein, TUM1) is encoded by the MPST (also known as TST2) gene (Gene ID 4357) in human. 3-mercaptopyruvate sulfurtransferase (MpST) catalyzes the transfer of a sulfur ion to cyanide or other thiol compounds and plays a central role in both cysteine degradation and cyanide detoxification. MpST is also known as tRNA thiouridine modification protein (TUM1) and is implicated in other cellular processes in addition to cyanide detoxification, ranging from thiolation of cytosolic tRNAs to the generation of H2S as signaling molecule both in mitochondria and the cytosol. Chronic cyanide intoxications caused by cyanogenic substances present in food products can lead to neurological disorders. MpST and rhodanese [EC 2.8.1.1] are two enzymes that catalyze cyanide detoxification, with MpST also displaying weak rhodanese activity. Cyanide detoxification by rhodanese requires the presence of compounds bearing labile sulfane sulfur, while mercaptopyruvate is needed for MpST catalyzed reaction. Under oxidative stress conditions, the catalytic cysteine site is converted to a sulfenate, resulting in an inactivation of MpST enzymatic activity. Reduced thioredoxin reactivates the enzyme by cleaving an intersubunit disulfide bond to turn the redox switch on. Aberrant MpST activity is found in a few cases of mercaptolactate-cysteine disulfiduria (MCDU) characterized by the appearance of large quantaties of the sulfur-containing amino acid, beta-mercaptolactate-cysteine disulfide, in the urine.
Specyficzność
Clone 1H10.1 targets an epitope in the second Rhodanese domain present in both spliced isoforms of human MPST reported by UniProt (P25325).
Immunogen
Epitope: Second Rhodanese domain.
GST-tagged recombinant fragment from the second Rhodanese domain of human MPST.
Zastosowanie
Research Category
Signaling
Signaling
Research Sub Category
Toxicology & Drug Resistance
Toxicology & Drug Resistance
This Anti-MPST Antibody, clone 1H10.1 is validated for use in Western Blotting, Immunohistochemistry (Paraffin) for the detection of MPST.
Western Blotting Analysis: 0.5 µg/mL from a representative lot detected MPST in 10 µg of human liver tissue lysate.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected MPST in human heart and kidney tissue sections.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected MPST in human heart and kidney tissue sections.
Jakość
Evaluated by Western Blotting in SHSY-5Y cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected MPST in 10 µg of SHSY-5Y cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected MPST in 10 µg of SHSY-5Y cell lysate.
Opis wartości docelowych
~33 kDa observed. 33.05 kDa (isoform 1) and 35.12 kDa (isoform 2) calculated (Met1 removed). Uncharacterized band(s) may appear in some lysates.
Postać fizyczna
Format: Purified
Protein G purified.
Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Przechowywanie i stabilność
Stable for 1 year at 2-8°C from date of receipt.
Inne uwagi
Concentration: Please refer to lot specific datasheet.
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania
12 - Non Combustible Liquids
Klasa zagrożenia wodnego (WGK)
WGK 1
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