MABN730
Anti-Rab10 Antibody, clone 4E2
ascites fluid, clone 4E2, from mouse
Synonim(y):
RAB10, Ras-related protein Rab-10, Rab10
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About This Item
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Polecane produkty
pochodzenie biologiczne
mouse
Poziom jakości
forma przeciwciała
ascites fluid
rodzaj przeciwciała
primary antibodies
klon
4E2, monoclonal
reaktywność gatunkowa
human, mouse
metody
immunofluorescence: suitable
western blot: suitable
izotyp
IgG1
Warunki transportu
wet ice
docelowa modyfikacja potranslacyjna
unmodified
informacje o genach
human ... RAB10(10890)
Opis ogólny
Ras related protein Rab-10 (Rab10) is encoded by the gene named RAB10 and is member of the small GTPase family of Rab proteins. Rab proteins play critical roles in intracellular trafficking from the formation of vesicles to their fusion with membranes. Specifically Rab10 is involved in regulating RME-1 in endocytic recycling as well as endoplasmic reticulum structural dynamics and morphology, and Rab10 also appears to be critical to formation and correct placement of proteins in such critical cellular structures as the basement membranes of during organ morphogenesis and basement membrane remodeling during development. Rab10 localizes to internal membrane structures like the endoplasmic reticulum and Golgi apparatus but in certain cells it can be found associated with cilia and other cellular structures as well. EMD-Millipore’s Anti-Rab10 mouse monoclonal antibody has been tested in western blot against human HeLa and mouse NIH3T3 cell lysates and in fluorescent immunocytochemistry on LOVO cells in culture.
Immunogen
Purified recombinant fragment of human Rab10 expressed in E. Coli.
Zastosowanie
Detect Ras related protein Rab-10 using this mouse monoclonal antibody, Anti-Rab10 Antibody, clone 4E2 validated for use in western blotting & Immunofluorescence.
Immunofluorescence Analysis: A 1:200-1,000 dilution from a representative lot detected Rab10 in LOVO cells.
Optimal working dilutions must be determined by end user.
Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Developmental Neuroscience
Developmental Neuroscience
Jakość
Evaluated by Western Blotting in HeLa and NIH/3T3 cell lysates.
Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected Rab10 in HeLa and NIH/3T3 cell lysates.
Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected Rab10 in HeLa and NIH/3T3 cell lysates.
Opis wartości docelowych
~25 kDa observed. Uncharacterized bands may appear in some lysate(s).
Postać fizyczna
Mouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.
Unpurified
Przechowywanie i stabilność
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Komentarz do analizy
Control
HeLa and NIH/3T3 cell lysates
HeLa and NIH/3T3 cell lysates
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania
12 - Non Combustible Liquids
Klasa zagrożenia wodnego (WGK)
nwg
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.
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