MABN389

Anti-α-Synuclein (SNCA) Antibody

mouse monoclonal, 5G4

• Synonim(y): Aggregated a-synuclein, Alpha-synuclein, Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor, NACP
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• Clone: 5G4, monoclonal
• Species reactivity: human
• Application: ELISA, IHC, WB
• Citations: 32

Właściwości

• Nazwa produktu: Anti-Aggregated a-Synuclein Antibody, clone 5G4, clone 5G4, from mouse, purified by affinity chromatography
• biological source: mouse
• Quality Segment: 100
• antibody form: affinity purified immunoglobulin
• antibody product type: primary antibodies
• clone: 5G4, monoclonal
• purified by: affinity chromatography
• species reactivity: human
• technique(s): ELISA: suitable, immunohistochemistry: suitable, western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_000336
• UniProt accession no.: P37840
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: human ... SNCA(6622)

Opis

General description

α-Synuclein, also known as Non-A beta component of AD amyloid or Non-A4 component of amyloid, or NACP, and encoded by the gene SNCA/NACP/PARK1, is a protein originally identified as non-Abeta component protein (NACP) in the amyloid enriched fraction isolated from Alzheimer’s brain tissue. α-Synuclein is expressed in neural tissue and non-neural tissues too. In neurons, α-Synuclein is localized to the nucleus, presynaptic termini and mitochondria of certain brain regions. α-Synuclein is a molecular chaperone that binds various proteins and compounds including synaptobrevin-2 protein and membrane phospholipids and is involved in vesicle trafficking and membrane transport within neurons. Natural, non-mutated α-Synuclein has autoproteolytic activity and naturally forms stable alpha helical tetramers. However, mutated α-Synuclein can aggregate to form insoluble pathological complexes such as Lewy Bodies inside neurons. Lewy Bodies also contain ubiquitin and it is thought that they are evidence of disrupted proteasome function with regards to α-synuclein but research is still continuing.
Pepscan epitope mapping experiments showed that the epitope of 5G4 seems to be hidden inside full-length a-synuclein in aqueous solution, and this fact could depend on a specific conformation of the molecule. Thus, after conformational change, like b-sheet building, the binding structure of 5G4 may be accessible in biological samples.
This antibody can be used as a sandwich ELISA pair with Cat. No. MABN633, Anti-a-Synuclein, clone 10D2.

~15 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.

Immunogen

KLH-conjugated linear peptide corresponding to human Aggregated a-Synuclein.

Application

Research Category
Neuroscience

Research Sub Category
Neurodegenerative Diseases

This Anti-Aggregated a-Synuclein Antibody, clone 5G4 is validated for use in western blotting, IHC & ELISA for the detection of Aggregated a-Synuclein.

Western Blotting Analysis: 2 µg/mL from a representative lot detected Aggregated a-Synuclein in human brain tissue lysate.

ELISA Analysis: A representative lot from an independent laboratory detected Aggregated a-Synuclein in a sandwich ELISA with Cat. No. MABN633, Anti-a-Synuclein, clone 5G4 (Kovacs, G. G., et al. (2012). Acta Neuropathol. 123(1):37-50.).

Physical form

Affinity purified

Purified mouse monoclonal IgG1κ in buffer containing PBS without preservatives.

Preparation Note

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Evaluated by Immunohistochemistry in brain tissue from a Parkinson′s disease patient.

Immunohistochemistry Analysis: A representative lot detected Aggregated a-Synuclein in brain tissue from a Parkinson′s disease patient.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Klasa składowania: 12 - Non Combustible Liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable