MABC524

Anti-FANCC Antibody, clone 8F3

clone 8F3, 1 mg/mL, from mouse

• Synonim(y): Fanconi anemia group C protein, Protein FACC, FANCC
• Kod UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Właściwości

• pochodzenie biologiczne: mouse
• Poziom jakości: 100
• forma przeciwciała: purified immunoglobulin
• rodzaj przeciwciała: primary antibodies
• klon: 8F3, monoclonal
• reaktywność gatunkowa: mouse, human, rat
• stężenie: 1 mg/mL
• metody: immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• izotyp: IgG2bκ
• numer dostępu NCBI: NP_000127
• numer dostępu UniProt: Q00597
• Warunki transportu: wet ice
• docelowa modyfikacja potranslacyjna: unmodified
• informacje o genach: human ... FANCC(2176)

Opis

Opis ogólny

Fanconi anemia (FA) is an inherited bone marrow failure syndrome associated with a progressive decline in hematopoietic stem cells, developmental defects, and predisposition to cancer. FANCC is part of the multisubunit Fanconi anemia (FA) complex composed of FANCA, FANCB, FANCC, FANCE, FANCF, FANCG, FANCL/PHF9 and FANCM. FANCC is a primarily nuclear localized DNA repair protein that is believed to function in interstrand DNA cross-link repair and the maintenance of normal chromosome stability. Mutations in FANCC are associated with various aspects of FA. New research shows that FANCC is critical for resistance to DNA cross-linking drug-induced DNA damage in cells patients with myeloproliferative neoplasms. Also, FANCC interacts with the C-terminal-binding protein-1 (CtBP1) involved in transcriptional regulation, including being a negative regulator of Dickkopf-1 (DKK1) expression. This down regulation does not occur in mutations of FANCC, which then leads to up-regulation of DKK1, a molecule involved in hematopoietic malignancies.

Immunogen

Linear peptide corresponding to Human FANCC.

Zastosowanie

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

This Anti-FANCC Antibody, clone 8F3 is validated for use in Western Blotting and Immunoprecipitation and Immunocytochemistry for the detection of FANCC.

Western Blotting Analysis: 4 µg/mL of a representative lot of this antibody detected FANCC in 10 µg of Mouse brain lysate.

Western Blotting Analysis: A representative lot detected FANCC in HeLa cell lysate (Tremblay, C.S., et al. (2008) Blood. 112(5):2062-2070).

Immunoprecipitation Analysis: A representative lot immunoprecipitated FANCC from HeLa cell lysate (Tremblay, C.S., et al. (2008) Blood. 112(5):2062-2070).

Immunocytochemistry Analysis: A representative lot detected FANCC in HeLa cells (Tremblay, C.S., et al. (2008) Blood. 112(5):2062-2070).

Jakość

Evaluated by Western Blotting in PC12 cell lysate.

Western Blotting Analysis: 4 µg/mL of this antibody detected FANCC in 10 µg of PC12 cell lysate.

Opis wartości docelowych

~ 63 kDa observed

Postać fizyczna

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Przechowywanie i stabilność

Stable for 1 year at 2-8°C from date of receipt.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable