MAB386
Anti-Myelin Basic Protein Antibody, a.a. 82-87
culture supernatant, clone 12, Chemicon®
Synonim(y):
MBP
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About This Item
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Polecane produkty
pochodzenie biologiczne
rat
Poziom jakości
forma przeciwciała
culture supernatant
rodzaj przeciwciała
primary antibodies
klon
12, monoclonal
reaktywność gatunkowa
human, rat, sheep, mouse, rabbit, chicken, guinea pig, bovine, all
producent / nazwa handlowa
Chemicon®
metody
ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
radioimmunoassay: suitable
western blot: suitable
izotyp
IgG2a
numer dostępu NCBI
numer dostępu UniProt
Warunki transportu
dry ice
docelowa modyfikacja potranslacyjna
unmodified
informacje o genach
human ... MBP(4155)
mouse ... Mbp(17196)
rat ... Mbp(24547)
Opis ogólny
The two major components of CNS myelin are Myelin Basic Protein (MBP) and proteolipid protein (PLP), occuring in roughly equimolar proportions. There are many neurological disorders associated with deficiencies of myelin assembly and structure. For example Multiple Sclerosis is a human disease due to multiple factors but generally characterized by the degradation of the myelin sheath. MBP ususally refers to the 18.5 kDa isoform, which is one of the most abundant proteins of the myelin sheath of adult human and bovine CNS. Genetically, MBP is the product of differential splicing of a single mRNA transcript. In humans there are 4 isoforms, 21.5, 20.2, 18.5, and 17.2 kDa. Thus, MBP are present in multiple isoforms, part of a large family of developmentally expressed, translocatable, and highly post-translationally modifed proteins with multiple binding partners. Structurally, MBP displays a predominately unorganized form with greater than 75% in random coil. Other non-CNS related functions of myelin include insulin induction and glucagon release from pancreatic islets.
Specyficzność
Reacts with MBP from all species tested including human, bovine, sheep, rabbit, mouse, rat, guinea pig and chicken.
Reacts with MBP from all species tested. It reacts weakly with peptides ending in the phe 91 where the 91-92 phe-phe bond is broken. Synthetic peptide 82-99 reacts very well as does intact MBP. Mapped to the region DENPVV.
Immunogen
Bovine myelin basic protein
Epitope: a.a. 82-87
Zastosowanie
Anti-Myelin Basic Protein Antibody, a.a. 82-87 is an antibody against Myelin Basic Protein for use in ELISA, IC, IH, RIA & WB.
Immunohistochemistry:
A previous lot of this antibody was used on frozen sections.
ELISA/RIA:
1:1000 dilution of a previous lot of this antibody was used in ELISA/RIA.
Western Blotting:
Identifies an 18-20kDa band in westerns.
Optimal working dilutions must be determined by end user.
A previous lot of this antibody was used on frozen sections.
ELISA/RIA:
1:1000 dilution of a previous lot of this antibody was used in ELISA/RIA.
Western Blotting:
Identifies an 18-20kDa band in westerns.
Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neuronal & Glial Markers
Neurochemistry & Neurotrophins
Neuronal & Glial Markers
Neurochemistry & Neurotrophins
Jakość
Evaluated by Western Blot on Mouse brain lysates.
Western Blot Analysis:
1:500 dilution of antibody lot detected four isoforms of MBP on 10 µg of Mouse brain lysates.
Western Blot Analysis:
1:500 dilution of antibody lot detected four isoforms of MBP on 10 µg of Mouse brain lysates.
Opis wartości docelowych
18-20 kDa
Postać fizyczna
Liquid containing 0.1% sodium azide.
Unpurified
Przechowywanie i stabilność
Stable for 1 year at -20ºC from date of receipt.
Komentarz do analizy
Control
Brain tissue
Brain tissue
Inne uwagi
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informacje prawne
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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polecane
Kod klasy składowania
12 - Non Combustible Liquids
Klasa zagrożenia wodnego (WGK)
nwg
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
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