MAB382
Anti-Myelin Basic Protein Antibody, a.a. 129-138, clone 1
culture supernatant, clone 1, Chemicon®
Synonim(y):
Myelin A1 protein, Myelin membrane encephalitogenic protein, myelin basic protein
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About This Item
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Polecane produkty
pochodzenie biologiczne
mouse
Poziom jakości
forma przeciwciała
culture supernatant
rodzaj przeciwciała
primary antibodies
klon
1, monoclonal
reaktywność gatunkowa
bovine, rat, rabbit (weakly), human
spodziewany brak reakcji z
guinea pig
producent / nazwa handlowa
Chemicon®
metody
ELISA: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
radioimmunoassay: suitable
western blot: suitable
izotyp
IgG2a
numer dostępu NCBI
numer dostępu UniProt
Warunki transportu
dry ice
docelowa modyfikacja potranslacyjna
unmodified
informacje o genach
human ... MBP(4155)
rat ... Mbp(24547)
Opis ogólny
The classic group of MBP isoforms (isoforms 4-14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoforms 1-3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined to optional posttranslational modifications give a wide spectrum of isomers, each of them having maybe a specialized function.
MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system.
Isoform 1 Golli-MBP1, HOG7, 33 kDa
Isoform 2 Golli-MBP2, HOG5, 21.5 kDa
Isoform 3 MBP1, 21.5 kDa
Isoform 4 MBP2, 20.2 kDa
Isoform 5 MBP3, 18.5 kDa
Isoform 6 MBP4, 17.2 kDa
(SP_P02686)
MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system.
Isoform 1 Golli-MBP1, HOG7, 33 kDa
Isoform 2 Golli-MBP2, HOG5, 21.5 kDa
Isoform 3 MBP1, 21.5 kDa
Isoform 4 MBP2, 20.2 kDa
Isoform 5 MBP3, 18.5 kDa
Isoform 6 MBP4, 17.2 kDa
(SP_P02686)
Specyficzność
Reacts with MBP from human, bovine and rat, epitope 129-138
Immunogen
Bovine myelin basic protein
Epitope: a.a. 129-138
Zastosowanie
Immunohistochemistry(paraffin) Analysis:
Optimal Staining With Citrate Buffer, pH 6.0, Epitope Retrieval: Rat Cerebellum
Immunohistology on frozen sections at 1:10
Western Blot Analysis:
A previous lot of this antibody was used in Western Blot.
ELISA:
A 1:200-1:1,000 dilution of a previous lot was used in ELISA.
RIA:
A previous lot of this antibody was used in Radioimmunoassay.
Optimal working dilutions must be determined by end user.
Optimal Staining With Citrate Buffer, pH 6.0, Epitope Retrieval: Rat Cerebellum
Immunohistology on frozen sections at 1:10
Western Blot Analysis:
A previous lot of this antibody was used in Western Blot.
ELISA:
A 1:200-1:1,000 dilution of a previous lot was used in ELISA.
RIA:
A previous lot of this antibody was used in Radioimmunoassay.
Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neuronal & Glial Markers
Neurochemistry & Neurotrophins
Neuronal & Glial Markers
Neurochemistry & Neurotrophins
This Anti-Myelin Basic Protein Antibody, a.a. 129-138, clone 1 is validated for use in ELISA, IH, IH(P), RIA, WB for the detection of Myelin Basic Protein.
Jakość
Routinely evaluated by immunohistochemistry on brain tissue.
Immunohistochemistry(paraffin) Analysis:
MBP (cat. # MAB382) staining pattern/morphology in rat cerebellum. Tissue pretreated with Citrate, pH 6.0. This lot of antibody was diluted to 1:50, using IHC-Select Detection with HRP-DAB. Immunoreactivity is seen as fiber staining in the junction between granular layer and molecular layer.
Optimal Staining With Citrate Buffer, pH 6.0, Epitope Retrieval: Rat Cerebellum
Immunohistochemistry(paraffin) Analysis:
MBP (cat. # MAB382) staining pattern/morphology in rat cerebellum. Tissue pretreated with Citrate, pH 6.0. This lot of antibody was diluted to 1:50, using IHC-Select Detection with HRP-DAB. Immunoreactivity is seen as fiber staining in the junction between granular layer and molecular layer.
Optimal Staining With Citrate Buffer, pH 6.0, Epitope Retrieval: Rat Cerebellum
Opis wartości docelowych
19 kDa
Postać fizyczna
Culture supernatant containing 0.2 M Tris/HCl, pH 7.4 with 5-10% fetal calf serum and 0.1% sodium azide
Unpurified
Przechowywanie i stabilność
Stable for 1 year at -20ºC in undiluted aliquots from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Komentarz do analizy
Control
Brain tissue
Brain tissue
Inne uwagi
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informacje prawne
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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polecane
Kod klasy składowania
10 - Combustible liquids
Klasa zagrożenia wodnego (WGK)
WGK 1
Certyfikaty analizy (CoA)
Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.
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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
Autoantibodies to myelin basic protein catalyze site-specific degradation of their antigen.
Ponomarenko, NA; Durova, OM; Vorobiev, II; Belogurov, AA; Kurkova, IN; Petrenko et al.
Proceedings of the National Academy of Sciences of the USA null
Michel Guipponi et al.
The American journal of pathology, 171(2), 608-616 (2007-07-11)
Defective proteolysis has been implicated in hearing loss through the discovery of mutations causing autosomal recessive nonsyndromic deafness in a type II transmembrane serine protease gene, TMPRSS3. To investigate their physiological function and the contribution of this family of proteases
Nucleus-localized 21.5-kDa myelin basic protein promotes oligodendrocyte proliferation and enhances neurite outgrowth in coculture, unlike the plasma membrane-associated 18.5-kDa isoform.
Smith, GS; Samborska, B; Hawley, SP; Klaiman, JM; Gillis, TE; Jones, N; Boggs, JM; Harauz, G
Journal of Neuroscience Research null
Brittney A Beyer et al.
Nature chemical biology, 14(1), 22-28 (2017-11-14)
Endogenous metabolites play essential roles in the regulation of cellular identity and activity. Here we have investigated the process of oligodendrocyte precursor cell (OPC) differentiation, a process that becomes limiting during progressive stages of demyelinating diseases, including multiple sclerosis, using
Chiara De Nuccio et al.
International journal of molecular sciences, 21(19) (2020-10-03)
An adequate protection from oxidative and inflammatory reactions, together with the promotion of oligodendrocyte progenitor (OP) differentiation, is needed to recover from myelin damage in demyelinating diseases. Mitochondria are targets of inflammatory and oxidative insults and are essential in oligodendrocyte
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