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MAB342

Sigma-Aldrich

Anti-Galactocerebroside Antibody, clone mGalC

clone mGalC, Chemicon®, from mouse

Synonim(y):

Galactoceramide, Galactocerebroside, Galactosylceramide, GalC

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About This Item

Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

pochodzenie biologiczne

mouse

Poziom jakości

forma przeciwciała

purified antibody

klon

mGalC, monoclonal

reaktywność gatunkowa

mouse, human, rabbit, bovine, chicken, rat

producent / nazwa handlowa

Chemicon®

metody

ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable

izotyp

IgG3

Warunki transportu

dry ice

docelowa modyfikacja potranslacyjna

unmodified

Opis ogólny

Galactocerebroside (GalC) is a major galactosphingolipid of myelin which plays a role in myelination. GalC is a very useful, specific marker for oligodendroglial lineage.

Specyficzność

Galactocerebroside (GalC), sulfatide, psychosine and other galactolipids. Cross-reacts with the sulfatide ester of GalC, but to a 16-fold lesser extent. No cross-reactivity with sphingosine, ceramide, mixed ganglioside or glucocerebroside. Binds specifically with oligodendrocytes and Schwann cells.

Immunogen

Synaptic plasma membranes from bovine hippocampus. Ranscht, B. et al. PNAS 79(8):2709-2713 (1982)

Zastosowanie

Anti-Galactocerebroside Antibody, clone mGalC is an antibody against Galactocerebroside for use in ELISA, IC, & IHC.
Immunohistochemistry:
0.5-10 μg/mL of a previous lot was used on formalin fixed frozen sections of a prevous lot. Can not be used on paraffin embedded tissue sections since the antigen is denatured during embedding and paraffin removal.

Immunocytochemistry:
0.5-10 μg/mL of a previous lot was used on 4% paraformaldehyde, acetic acid or ethanol fixed cultured cells.

ELISA:
A previous lot was used on purified galactocerebrosides.

Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Research Sub Category
Neuronal & Glial Markers

Postać fizyczna

Format: Purified
Protein A purified
Purified mouse monoclonal in buffer containing 10 mM Potassium Phosphate, 150 mM NaCl, pH 7.4 containing 0.09% sodium azide.

Przechowywanie i stabilność

Stable for 1 year at -20ºC from date of receipt.

Komentarz do analizy

Control
Neonatal cortex

Inne uwagi

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informacje prawne

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania

12 - Non Combustible Liquids

Klasa zagrożenia wodnego (WGK)

WGK 1

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable


Certyfikaty analizy (CoA)

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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Oligodendrocyte dysfunction after induction of experimental anterior optic nerve ischemia.
Goldenberg-Cohen, N; Guo, Y; Margolis, F; Cohen, Y; Miller, NR; Bernstein, SL
Investigative Ophthalmology & Visual Science null
HIV binding, penetration, and primary infection in human cervicovaginal tissue.
Diane Maher, Xiaoyun Wu, Timothy Schacker, Julie Horbul, Peter Southern
Proceedings of the National Academy of Sciences of the USA null
Adaptive HIV-specific B cell-derived humoral immune defenses of the intestinal mucosa in children exposed to HIV via breast-feeding.
Moussa, S; Jenabian, MA; Gody, JC; Leal, J; Gresenguet, G; Le Faou, A; Belec, L
Testing null
T Matsushita et al.
Neuroscience, 136(1), 115-121 (2005-09-27)
The successive stages of development from oligodendrocyte progenitor to mature oligodendrocyte have been investigated in detail by using stage-specific antibodies. However, no cell lines are available that show stepwise differentiation from oligodendrocyte progenitors to mature oligodendrocytes. Here we show the
Shih-Chieh Hung et al.
Stem cells (Dayton, Ohio), 20(6), 522-529 (2002-11-29)
Size-sieved stem (SS) cells isolated from human bone marrow and propagated in vitro are a population of cells with consistent marker typing, and can form bone, fat, and cartilage. In this experiment, we demonstrated that SS cells could be induced

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