MAB1527

Anti-Peripherin Antibody, clone 8G2

purified antibody, Chemicon^®

• Synonim(y): Anti-NEF4, Anti-PRPH1
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Uniprot accession no.: P41219
• Application: ICC, IHC, WB
• Citations: 55

Właściwości

• biological source: mouse
• Quality Level: 100, 300
• conjugate: unconjugated
• antibody form: (Purified at 1mg/ml in PBS and glycerol (1:1) with 5mM sodium azide)
• antibody product type: primary antibodies
• clone: 8G2, monoclonal
• form: (Liquid)
• species reactivity: pig, rat, bovine, human, mouse
• manufacturer/tradename: Chemicon^®
• technique(s): immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable
• isotype: IgG
• suitability: not suitable for immunohistochemistry (Paraffin)
• NCBI accession no.: NM_006262.3
• UniProt accession no.: P41219
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: human ... PRPH2(5961)

Opis

General description

Peripherin is a ~57kDa intermediate filament subunit found initially in sensory neurons of the peripheral nervous systems. Subsequently it was found in some sensory and other neurons of the central nervous system and also in PC12 cells. Peripherin is also expressed in certain neuroendocrine tumors and in the insulin producing cells of the pancreas. Peripherin belongs to the Class III family of intermediate filament subunits which includes vimentin, glial fibrillary acidic protein (GFAP) and desmin. Antibodies to peripherin can be used in identifying, classifying, and studying neurons throughout the nervous system. Peripherin is also a good diagnostic marker for ballooned axons seen in Lou Gehrig′s disease (Amyotrophic lateral sclerosis) and some neuronally derived tumors. Autoantibodies to peripherin are frequently seen in the sera of patients with diabetes. Peripherin is not related to peripherin/RDS, a protein of the photoreceptor outer membrane mutations of which are causative of certain forms of slow retinal degeneration.

57kDa

Immunogen

Electrophoretically pure trp-E-peripherin fusion protein [Dev. Brain Res. (1990). 57:235-248] containing all but the 4 N-terminal amino acids of rat peripherin. Fusion protein purified from bacterial inclusion bodies by DEAE-cellulose chromatography in 6M urea followed by preparative SDS-PAGE

Application

Anti-Peripherin Antibody, clone 8G2 is an antibody against Peripherin for use in IC, IH & WB.

Immunocytochemistry: 4% PFA fixed cells (5 minutes) or acetone is recommended.

Immunohistochemisty: light PFA fixation is necessary as 8G2 is sensitive to heavy formalin fixation. Antigen recovery is enhanced with 0.1% triton in the block only. 1:25-1:200

Immunoblotting: The antibody is clean and specific for the expected 57kDa band on Western blots.

Optimal working dilutions must be determined by end user.

Research Category
Neuroscience

Research Sub Category
Sensory & PNS

Neuronal & Glial Markers

Biochem/physiol Actions

Recognizes Peripherin. Strong staining on rat, mouse, human, pig and cow peripherin. Does not work on chicken or other more distantly related species, which may lack peripherin.

Physical form

UnPurified mouse culture supernatant containing no preservatives.

Unpurified

Preparation Note

Maintain for 12 months at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Rat sensory neurons, rat spinal cord homogenate and peripheral nerve homogenate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Klasa składowania: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable