MAB3408
Anti-Tubulin Antibody, beta, clone KMX-1
clone KMX-1, Chemicon®, from mouse
Synonim(y):
Tubulin beta-III, tubulin, beta 3, tubulin, beta 4
Zaloguj sięWyświetlanie cen organizacyjnych i kontraktowych
About This Item
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Polecane produkty
pochodzenie biologiczne
mouse
Poziom jakości
forma przeciwciała
purified immunoglobulin
rodzaj przeciwciała
primary antibodies
klon
KMX-1, monoclonal
reaktywność gatunkowa
human, rat, mouse
reaktywność gatunkowa (przewidywana na podstawie homologii)
all
producent / nazwa handlowa
Chemicon®
metody
immunocytochemistry: suitable
western blot: suitable
izotyp
IgG2b
numer dostępu NCBI
numer dostępu UniProt
Warunki transportu
wet ice
docelowa modyfikacja potranslacyjna
unmodified
informacje o genach
human ... TUBB3(10381)
Opis ogólny
Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55,000 kDa. Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of beta Tubulin may not be stable in certain cells. For example, expression of tubulin in adipose tissue is very low and therefore beta Tubulin should not be used as loading control for these tissues.
Specyficzność
This antibody reacts specifically with β-tubulin and shows a strong cross-reactivity to different species. This antibody allows staining of microtubuli using indirect immunofluorescence showing a low background only.
Immunogen
Tubulin purified from polycephalum myxamoebae
Zastosowanie
Immunocytochemistry:
A 1-2 μg/mL concentration of a previous lot was used in immunocytochemistry.
Western blot:
0.05-0.5 μg/mL
Optimal working dilutions must be determined by end user.
A 1-2 μg/mL concentration of a previous lot was used in immunocytochemistry.
Western blot:
0.05-0.5 μg/mL
Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Cytoskeleton
Cytoskeleton
This Anti-Tubulin Antibody, beta, clone KMX-1 is validated for use in IC, WB for the detection of Tubulin.
Jakość
Routinely evaluated by Western Blot on A431 lysates.
Western blot:
1:500 dilution of this lot detected TUBULIN (BETA) on 10 μg of A431 lysates.
Western blot:
1:500 dilution of this lot detected TUBULIN (BETA) on 10 μg of A431 lysates.
Opis wartości docelowych
50 kDa
Powiązanie
Replaces: 04-1049
Postać fizyczna
Format: Purified
Protein A purified
Purified mouse monoclonal IgG2b liquid in buffer containing 0.02M phosphate buffer, 0.25M NaCl with 0.1% sodium azide.
Przechowywanie i stabilność
Stable for 6 months at 2-8ºC in undiluted aliquots from date of receipt.
Komentarz do analizy
Control
HeLa Cell lysate
A431 Cell lysate
MCF7 Cell lysate
HeLa Cell lysate
A431 Cell lysate
MCF7 Cell lysate
Inne uwagi
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informacje prawne
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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polecane
Kod klasy składowania
12 - Non Combustible Liquids
Klasa zagrożenia wodnego (WGK)
WGK 2
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.
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