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MAB1637

Sigma-Aldrich

Anti-Tubulin Antibody, beta III isoform, CT, clone TU-20 (Similar to TUJ1)

ascites fluid, clone TU-20 (Similar to TUJ1), Chemicon®

Synonim(y):

Tubulin beta-III, tubulin, beta 3, tubulin, beta 4

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About This Item

Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

pochodzenie biologiczne

mouse

Poziom jakości

100

forma przeciwciała

ascites fluid

rodzaj przeciwciała

primary antibodies

klon

TU-20 (Similar to TUJ1), monoclonal

reaktywność gatunkowa

rat, mouse, avian, human, bovine, monkey, pig, sheep

producent / nazwa handlowa

Chemicon®

metody

ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable
western blot: suitable

izotyp

IgG1

numer dostępu NCBI

NM_006086.2

numer dostępu UniProt

Q13509

Warunki transportu

dry ice

docelowa modyfikacja potranslacyjna

unmodified

informacje o genach

human ... TUBB3(10381)

Opis ogólny

Tubulin is one of several members of a small family of globular proteins. The most common members of the tubulin family are α-tubulin and β-tubulin, the proteins which makes up microtubules. Each has a molecular weight of approximately 55 kDa. Microtubules are assembled from dimers of α- and β-tubulin. These subunits are slightly acidic with an isoelectric point between 5.2 and 5.8. Tubulin was long thought to be specific to eukaryotes. Recently, however, the prokaryotic cell division protein FtsZ was shown to be evolutionarily related to tubulin.

Specyficzność

Expected to cross-react with Avian, Porcine (Pig), Rat, Bovine, Sheep, Human, Monkey. Reactivity with other species has not been confirmed.
The antibody reacts with the C-terminus of the neuron specific beta III isoform of tubulin. Does not identify beta tubulin found in glial cells.

Immunogen

A synthetic peptide corresponding to amino acids 443-450 (ESEAQGPK) of human class III beta-tubulin conjugated to KLH.
Epitope: C-terminus

Zastosowanie

Anti-Tubulin Antibody, beta III isoform, C-terminus, clone TU-20 (Similar to TUJ1) is an antibody against Tubulin for use in ELISA, IC, IH, IH(P), IP & WB with more than 55 product citations.
Immunohistochemistry:
On formalin fixed frozen or paraffin embedded tissue sections: 1:20 to 1:100 dilution from a previous lot. High temperature citrate buffer antigen retrieval is recommended with paraffin embedded tissue sections.

Immunoprecipitation:
A previous lot of this antibody was used on Immunoprecipitation.

Immunocytochemistry:
1:100 to 1:500 dilution from a previous lot was used on fixed cells.

Immunoblotting:
1:1000. Antibody will detect a single band at 50 kDa corresponding to class III beta-tubulin.

ELISA:
A previous lot of this antibody was used on ELISA.

Optimal working dilution must be determined by the end user.
Research Category
Neuroscience
Research Sub Category
Neuronal & Glial Markers

Jakość

Routinely evaluated by Western Blot on Mouse Brain lysates.

Western Blot Analysis:
1:1000 dilution of this lot detected tubulin (βIII) on 10 μg of Mouse Brain lysates.

Opis wartości docelowych

55 kDa

Powiązanie

Replaces: 04-1049

Postać fizyczna

Mouse monoclonal IgG1 ascites in buffer containing PBS containing 1% BSA and 15 mM sodium azide.
Unpurified

Przechowywanie i stabilność

Stable for 1 year at -20°C in undiluted aliquots from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Komentarz do analizy

Control
Positive control: Brain tissue or neurons of any type.
Negative control: Non neural tissue or Astrocytes.

Inne uwagi

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Informacje prawne

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania

12 - Non Combustible Liquids

Klasa zagrożenia wodnego (WGK)

WGK 2

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

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Odwiedź Bibliotekę dokumentów

Function of mouse embryonic stem cell-derived supporting cells in neural progenitor cell maturation and long term expansion.
Guan, Y; Du, QA; Zhu, W; Zou, C; Wu, D; Chen, L; Zhang, YA
Testing null
Shelley R Hough et al.
PloS one, 4(11), e7708-e7708 (2009-11-06)
Commitment in embryonic stem cells is often depicted as a binary choice between alternate cell states, pluripotency and specification to a particular germ layer or extraembryonic lineage. However, close examination of human ES cell cultures has revealed significant heterogeneity in
Andrew Campbell et al.
Glia, 64(2), 227-239 (2015-10-16)
Astroglial dysfunction plays an important role in neurodegenerative diseases otherwise attributed to neuronal loss of function. Here we focus on the role of astroglia in ataxia-telangiectasia (A-T), a disease caused by mutations in the ataxia-telangiectasia mutated (ATM) gene. A hallmark
Yoshitaka Uno et al.
Human gene therapy, 22(6), 711-719 (2010-12-21)
We originally reported the use of vitamin E (α-tocopherol) as an in vivo vector of short-interfering RNA (siRNA) to the liver. Here, we apply our strategy to the brain. By combining high-density lipoprotein (HDL) as a second carrier with α-tocopherol-conjugated
Agnese Po et al.
The EMBO journal, 29(15), 2646-2658 (2010-06-29)
Hedgehog (Hh) pathway has a pivotal function in development and tumorigenesis, processes sustained by stem cells (SCs). The transcription factor Nanog controls stemness acting as a key determinant of both embryonic SC self-renewal and differentiated somatic cells reprogramming to pluripotency
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Produkty

ReNcell® Human Neural Progenitors: Renewable and Consistent Human Neural Stem Cell Lines that Differentiate into Functional Neurons

ReNcell neural progenitors are immortalized human neural stem cell lines that can differentiate into neurons, astrocytes sand oligodendrocytes.

Derivation of Functional Oligodendrocyte Progenitor Cells (OPCs) from Human Neural Stem Cell Lines

Derivation and characterization of functional human neural stem cell derived oligodendrocyte progenitor cells (OPCs) that efficiently myelinate primary neurons in culture.

Uzyskanie funkcjonalnych komórek progenitorowych oligodendrocytów (OPC) z linii ludzkich nerwowych komórek macierzystych

Uzyskanie i scharakteryzowanie funkcjonalnych komórek progenitorowych oligodendrocytów (OPC) pochodzących z ludzkich neuronalnych komórek macierzystych, które skutecznie mielinizują pierwotne neurony w hodowli.

Protokoły

Neural Stem Cell Culture Protocols

Step-by-step culture protocols for neural stem cell culture including NSC isolation, expansion, differentiation and characterization.

Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.

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