MAB2030

Anti-CSPG4 Antibody

CHEMICON^®, mouse monoclonal, BE-123

• Synonim(y): Anti-CSPG4A, Anti-HMW-MAA, Anti-MCSP, Anti-MCSPG, Anti-MEL-CSPG, Anti-MSK16, Anti-NG2
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• Clone: BE-123, monoclonal
• Species reactivity: human, canine, rat, sheep, bovine, mouse
• Application: ELISA, RIA, WB
• Citations: 43

Właściwości

• Nazwa produktu: Anti-Chondroitin 4 Sulfate Antibody, clone BE-123, ascites fluid, clone BE-123, Chemicon^®
• biological source: mouse
• Quality Segment: 100
• antibody form: ascites fluid
• antibody product type: primary antibodies
• clone: BE-123, monoclonal
• species reactivity: human, canine, rat, sheep, bovine, mouse
• manufacturer/tradename: Chemicon^®
• technique(s): ELISA: suitable, radioimmunoassay: suitable, western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NM_001897.4
• UniProt accession no.: Q6UVK1
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: human ... CSPG4(1464)

Opis

Immunogen

Mouse proteoglycan.

Application

Anti-Chondroitin 4 Sulfate Antibody, clone BE-123 detects level of Chondroitin 4 Sulfate & has been published & validated for use in ELISA, RIA & WB.

ELISA at 1:400-1:3,200.

RIA at 1:50-1:6,400.

Immunohistochemistry: This antibody only reacts with digested material. Fixed tissue must be digested with chondroitinase ABC (Sigma).
The Reference 23757342 should be indicated for IHC. (Reine, TM., et al., PubMed ID: 23757342)

Western Blot: 1:1000-1:4000 using ECL detection method. Chondroitinase ABC treatment of lysate sample is helpful.
In any alkali buffer, e.g. Tris or acetate (50mM or 100mM) pH 7.4-7.6. Make a stock solution by diluting one vial of Sigma Chondroitinase A, B, C, (5 units/vial) in 100 µL . For use, dilute stock 10-fold into the buffer of choice. Use at a ratio of 50-100mg of proteoglycan per 5 units of enzyme. On fixed cells, dilute in Tris acetate and on non-fixed cellsy dilute 10-25 fold in DMEM.
Poole, C.A., et al., J. Histochem. Cytochem. 1991. 39:1175-1187.

Optimal working dilutions must be determined by the end user.

Research Category
Cell Structure

Research Sub Category
ECM Proteins

Biochem/physiol Actions

Reacts with both mouse (fetal and adult) and human (fetal and adult) chondroitinase ABC digested cartilage proteoglycans. Chondroitin-4 sulfate disaccharides successfully inhibit the binding of monoclonal antibody to proteoglycans. No reaction with native proteoglycans or with proteoglycans digested with either chondroitinase AC or testicular hyaluronidase.

Physical form

Ascites fluid containing no preservatives.

Preparation Note

Maintain at -20°C in undiluted aliquots for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Klasa składowania: 10 - Combustible liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable