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PureProteome Protein A Magnetic Bead System

The PureProteome Protein A Magnetic Bead System is a powerful system that helps researchers purify proteins by maximizing recovery and eliminating variability

Synonim(y):

Protein A Magnetic Beads

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Gabaryty przesyłkiSKUDostępnośćCena netto
2 x 1 mL

Dostępne do wysyłki DZISIAJzKuehne + Nagel Sp. z o.o.

1420,00 zł
10 mL

Dostępne do wysyłki DZISIAJzKuehne + Nagel Sp. z o.o.

4350,00 zł

Informacje o tej pozycji

UNSPSC Code:
41105507
NACRES:
NA.56
eCl@ss:
32160405

1420,00 zł


Dostępne do wysyłki DZISIAJSzczegóły


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Quality Level

packaging

pkg of 2 × 1 mL

manufacturer/tradename

PureProteome

technique(s)

depletion: suitable (serum), immunoprecipitation (IP): suitable, protein purification: suitable

particle size

10 μm

capacity

1.5-2.5 μg/μL, suspension binding capacity (rabbit IgG)

shipped in

wet ice

General description

The PureProteome Protein A Magnetic Bead System is a powerful system that helps researchers in protein purification by maximizing recovery and reducing variability.

Application

PureProteome Protein A Magnetic Bead System may be used:
  • in serum depletion, in immunoprecipitation, for protein purification, and in cell lysates to analyze the bound proteins by western blot[1]
  • in magnetic bead-binding assays[1]
  • for immunoprecipitating cell lysate and antibodies for ATPase activity[1]

Legal Information

PureProteome is a trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Ta pozycja
LSKMAGGLSKMAGHLSKMAGAG
particle size

10 μm

particle size

10 μm

particle size

10 μm

particle size

10 μm

manufacturer/tradename

PureProteome

manufacturer/tradename

PureProteome

manufacturer/tradename

PureProteome

manufacturer/tradename

PureProteome

packaging

pkg of 2 × 1 mL

packaging

pkg of 2 × 1 mL

packaging

pkg of 10 mL

packaging

pkg of 2 × 1 mL

capacity

1.5-2.5 μg/μL, suspension binding capacity (rabbit IgG)

capacity

2.5-3.5 μg/μL, suspension binding capacity (rabbit IgG)

capacity

1-5.5 mg/mL, bead suspension binding capacity (protein)

capacity

2-3 mg/mL, suspension binding capacity (rabbit IgG)

shipped in

wet ice

shipped in

wet ice

shipped in

ambient

shipped in

wet ice

technique(s)

depletion: suitable (serum), protein purification: suitable, immunoprecipitation (IP): suitable

technique(s)

depletion: suitable (serum), immunoprecipitation (IP): suitable, protein purification: suitable

technique(s)

protein purification: suitable (histidine tagged recombinant protein)

technique(s)

immunoprecipitation (IP): suitable (crosslinking), protein purification: suitable (antibody)


Klasa składowania

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Skin Sens. 1



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Produkty

Możliwe przyczyny i potencjalne środki zaradcze dla wyzwań napotykanych w technice immunoprecypitacji-Western blot, która składa się z lizy komórek, tworzenia kompleksu przeciwciało-antygen (immunologicznego), wytrącania kompleksów immunologicznych i analizy metodą Western blot.

PureProteome™ Protein A and G Magnetic beads provide a rapid and reproducible means to purify immunoglobulins (IgG) using the KingFisher Duo particle processor.

The possible causes and potential remedies for challenges encountered in the immunoprecipitation-Western blot technique, which consists of cell lysis, formation of the antibody-antigen (immune) complex, precipitation of the immune complexes, and analysis by Western blotting.

Powiązane treści

Find protein research tools to prepare, isolate, and analyze proteins. Organized by how to extract, protect, purify, enrich, modify, and quantify proteins.

Immunoprecipitation (IP) is a powerful technique for proteomic screening, biomarker discovery, and signaling network elucidation. It is frequently used to enrich target proteins from complex samples such as cell lysates or extracts. Traditional IP protocols use Protein A, Protein G or a mixture of Protein A and G coupled to a solid support resin, such as agarose beads, to capture an antigen/antibody complex in solution. As the number of samples increase, the traditional, manual IP method can be time-consuming. Processing of multiple IP reactions in parallel can introduce complexity, variability and pipetting errors, which may affect reproducibility.

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ACS medicinal chemistry letters, 8(3), 361-365 (2017-03-25)
Nitric oxide (NO) release from a suitable NO photodonor (NOP) can be fine-tuned by visible light stimuli at doses that are not toxic to cells but that inhibit several efflux pumps; these are mainly responsible for the multidrug resistance of
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Journal of biomolecular screening, 20(2), 292-298 (2014-10-10)
Human kynurenine 3-monooxygenase (KMO) is emerging as an important drug target enzyme in a number of inflammatory and neurodegenerative disease states. Recombinant protein production of KMO, and therefore discovery of KMO ligands, is challenging due to a large membrane targeting
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Biotechnology and bioengineering, 115(10), 2530-2540 (2018-05-20)
An increasing number of nonantibody format proteins are entering clinical development. However, one of the major hurdles for the production of nonantibody glycoproteins is host cell-related proteolytic degradation, which can drastically impact developability and timelines of pipeline projects. Chinese hamster



Numer pozycji handlu globalnego

SKUNUMER GTIN
LSKMAGA1004053252408557
LSKMAGA0204053252674846

Questions

  1. What is the concentration of the beads?

    1 answer
    1. The beads are not characterized in terms of beads/ml but only in terms of a percentage slurry (10%, weight [g] by volume [mL]).

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