KAA002
Cellular Senescence Assay
Cellular Senescence Assay Kits provide all the reagents required to efficiently detect SA-β-gal activity at pH 6.0 in cultured cells & tissue sections.
Synonim(y):
Senescence Detection Kit
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| Gabaryty przesyłki | SKU | Dostępność | Cena netto |
|---|---|---|---|
| 1 kit | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 1500,00 zł |
Informacje o tej pozycji
UNSPSC Code:
12161503
NACRES:
NA.84
eCl@ss:
32161000
1500,00 zł
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
Pomoc techniczna
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Pozwól nam pomócQuality Level
manufacturer/tradename
Chemicon®
technique(s)
cell based assay: suitable
detection method
colorimetric
shipped in
wet ice
General description
Cellular senescence is one of the most fundamental aspects of cell behavior, and is thought to play a critical role in regulating cellular lifespan both in vitro and in vivo [1-3]. Primary somatic cells grown in vitro do not proliferate indefinitely. Instead, after a period of rapid proliferation, cell division rate slows, and ultimately ceases altogether, with the cells becoming unresponsive to mitogenic stimuli. This process is termed cellular senescence, and senescent cells have a well-defined accompanying phenotype - increased cell size, distinctive flat morphology, accumulated lipofuscin granules, wide changes in gene expression, and activity of senescence-associated β-galactosidase (SA-β-gal) [2,3].
It is generally believed that cellular senescence reflects some of the changes that occur during the aging of organisms, and senescent cells have been detected in vivo at sites of age-related pathology, such as benign hyperplastic prostate [4] and atherosclerotic lesions [5]. Recent studies have also provided convincing demonstrations of cellular senescence occurring in vivo in response to internally- and externally-induced stress signals [6,7]. In all of these studies, senescence was characterized by the appearance of senescence-associated β-galactosidase (SA-β-gal) activity, in common with the senescent phenotype in vitro.
Cellular senescence has become an increasingly important target in the development of novel therapeutics. Emerging data implicates senescence bypass in the development of cancer and suggests that senescence may represent a tumor suppressor mechanism. The demonstration that tumor cells can be induced to undergo replicative senescence following the introduction of negative cell-cycle regulators, anti-telomerase peptides, or drug treatment suggests that induction of senescence can be exploited as a basis for cancer therapy [8,9].
For Research Use Only; Not for use in diagnostic procedures
Test Principle:
As outlined above, a classic characteristic of the senescent phenotype is the induction of senescence-associated β-galactosidase (SA-β-gal) activity. SA-β-gal is present only in senescent cells, not in presenescent, quiescent, or proliferating cells. Chemicon′s Cellular Senescence Assay Kit provides all the reagents required to efficiently detect SA-β-gal activity at pH 6.0 in cultured cells and tissue sections. In this assay, SA-β-gal catalyzes the hydrolysis of X-gal, which results in the accumulation of a distinctive blue color in senescent cells. Each kit provides sufficient quantities of reagents to perform at least 50 assays in 35 mm wells.
It is generally believed that cellular senescence reflects some of the changes that occur during the aging of organisms, and senescent cells have been detected in vivo at sites of age-related pathology, such as benign hyperplastic prostate [4] and atherosclerotic lesions [5]. Recent studies have also provided convincing demonstrations of cellular senescence occurring in vivo in response to internally- and externally-induced stress signals [6,7]. In all of these studies, senescence was characterized by the appearance of senescence-associated β-galactosidase (SA-β-gal) activity, in common with the senescent phenotype in vitro.
Cellular senescence has become an increasingly important target in the development of novel therapeutics. Emerging data implicates senescence bypass in the development of cancer and suggests that senescence may represent a tumor suppressor mechanism. The demonstration that tumor cells can be induced to undergo replicative senescence following the introduction of negative cell-cycle regulators, anti-telomerase peptides, or drug treatment suggests that induction of senescence can be exploited as a basis for cancer therapy [8,9].
For Research Use Only; Not for use in diagnostic procedures
Test Principle:
As outlined above, a classic characteristic of the senescent phenotype is the induction of senescence-associated β-galactosidase (SA-β-gal) activity. SA-β-gal is present only in senescent cells, not in presenescent, quiescent, or proliferating cells. Chemicon′s Cellular Senescence Assay Kit provides all the reagents required to efficiently detect SA-β-gal activity at pH 6.0 in cultured cells and tissue sections. In this assay, SA-β-gal catalyzes the hydrolysis of X-gal, which results in the accumulation of a distinctive blue color in senescent cells. Each kit provides sufficient quantities of reagents to perform at least 50 assays in 35 mm wells.
Application
Cellular Senescence Assay Kits provide all the reagents required to efficiently detect SA-β-gal activity at pH 6.0 in cultured cells & tissue sections.
Preparation Note
Store X-gal solution protected from light at -20°C, and other kit components at 4°C. All components supplied are stable for 1 year.
Precautions:
Please refer to the Material Safety Data Sheet at www.chemicon.com for any necessary precautions.
Precautions:
Please refer to the Material Safety Data Sheet at www.chemicon.com for any necessary precautions.
Other Notes
100X Fixing Solution: (Part No. 2004755) One 1.5 mL vial
10X Staining Solution A: (Part No. 2004756) One 15 mL bottle
10X Staining Solution B: (Part No. 2004754) One 15 mL bottle
X-gal Solution: (Part No. 2004752) Two 1.5 mL vials
10X Staining Solution A: (Part No. 2004756) One 15 mL bottle
10X Staining Solution B: (Part No. 2004754) One 15 mL bottle
X-gal Solution: (Part No. 2004752) Two 1.5 mL vials
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Ta pozycja | |||
|---|---|---|---|
| technique(s) cell based assay: suitable | technique(s) - | technique(s) - | technique(s) cell based assay: suitable |
| shipped in wet ice | shipped in dry ice | shipped in wet ice | shipped in wet ice |
| Quality Level 100 | Quality Level 200 | Quality Level 200 | Quality Level 100 |
| manufacturer/tradename Chemicon® | manufacturer/tradename - | manufacturer/tradename - | manufacturer/tradename Chemicon® |
| detection method colorimetric | detection method colorimetric | detection method colorimetric | detection method colorimetric |
signalword
Danger
Hazard Classifications
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Flam. Liq. 3 - Repr. 1B - Resp. Sens. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 3
target_organs
Respiratory system
supp_hazards
Klasa składowania
3 - Flammable liquids
flash_point_f
135.5 °F
flash_point_c
57.5 °C
wgk
WGK 3
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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
J Choi et al.
Urology, 56(1), 160-166 (2000-06-28)
Cellular senescence is a unique cellular response pathway thought to be closely associated with the aging process. The senescent phenotype is characterized by the loss of a cell's ability to respond to proliferative and apoptotic stimuli even while normal metabolic
Richard Marcotte et al.
The journals of gerontology. Series A, Biological sciences and medical sciences, 57(7), B257-B269 (2002-06-27)
Forty years after its discovery, replicative senescence remains a rich source of information about cell-cycle regulation and the progression from a normal to a transformed phenotype. Effectors of this growth-arrested state are being discovered at a great pace. This review
A Satyanarayana et al.
The EMBO journal, 22(15), 4003-4013 (2003-07-26)
Telomere shortening limits the regenerative capacity of primary cells in vitro by inducing cellular senescence characterized by a permanent growth arrest of cells with critically short telomeres. To test whether this in vitro model of cellular senescence applies to impaired
Numer pozycji handlu globalnego
| SKU | NUMER GTIN |
|---|---|
| KAA002 | 04053252009907 |