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CS0030

Sigma-Aldrich

Senescence Cells Histochemical Staining Kit

sufficient for 100 tests

Synonim(y):

Senescent cells IHC kit, cellular β-galactosidase probe

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About This Item

Kod UNSPSC:
12352207
NACRES:
NA.32

zastosowanie

sufficient for 100 tests

Poziom jakości

200

opakowanie

pkg of 1 kit

warunki przechowywania

dry at room temperature

metoda wykrywania

colorimetric

Warunki transportu

dry ice

temp. przechowywania

−20°C

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Opis ogólny

The Senescence Cells Histochemical Staining Kit contains all the reagents required for identifying senescent cells using an assay based on a histochemical stain for ß-galactosidase activity at pH 6. ß-galactosidase activity is detectable in senescent cells, but not in quiescent, immortal, or tumor cells.
The Senescence Cells Histochemical Staining Kit contains all the reagents required for identifying senescent cells using an assay based on a histochemical stain for β-galactosidase activity at pH 6. β-galactosidase activity is detectable in senescent cells, but not in quiescent, immortal, or tumor cells. Cellular senescence is a progression of events, whereby cells move from an actively dividing to a non-dividing stage. The cellular senescence process is associated with aging. The decrease in cell division is virtually irreversible and complete. In conjunction with the loss of the ability to divide, changes occur in the morphology, shape, and physical appearance of the cells, and their pattern of gene expression. At the end of the process cell death usually occurs, although the cells may remain viable for a long time.

Zastosowanie

Senescence Cells Histochemical Staining Kit has been used for:
  • senescence-associated β-galactosidase assay
  • to test for senescence in human pancreatic cancer cells
  • adipose-derived stem cells (ADSCs)
  • cord blood mesenchymal stromal/stem cells (CBMSC)

Działania biochem./fizjol.

Replicative senescence is a growth-arrest state associated with loss of division potential, changes in cell morphology, shape and physical appearance, and the pattern of gene expression in cells.

Informacje prawne

Manufactured under license to US Patent Nos. 5,491,069 and 5,795,728.

Tylko elementy zestawu

Numer produktu
Opis
  • X-gal solution 4 mL
  • Staining Solution, 10X 15 mL
  • Fixation Buffer 10x 15 mL
  • Reagent B 1.5 mL
  • Reagent C 1.5 mL
  • Dulbecco's Phosphate Buffered Saline (PBS) 10x 60 mL

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Hasło ostrzegawcze

Danger

Zwroty wskazujące rodzaj zagrożenia

H302 + H312,H314,H317,H331,H334,H335,H341,H350,H412

Klasyfikacja zagrożeń

Acute Tox. 3 Inhalation - Acute Tox. 4 Dermal - Acute Tox. 4 Oral - Aquatic Chronic 3 - Carc. 1B - Eye Dam. 1 - Muta. 2 - Resp. Sens. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 3

Organy docelowe

Respiratory system

Zagrożenia dodatkowe

EUH032

Kod klasy składowania

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

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B van der Loo et al.
Experimental cell research, 241(2), 309-315 (1998-06-25)
A beta-galactosidase activity has recently been used as a histochemical marker of replicative senescence in human fibroblasts and keratinocytes. To establish whether this marker could be used to detect senescence of vascular cells, we have investigated its presence in cultures
G P Dimri et al.
Proceedings of the National Academy of Sciences of the United States of America, 92(20), 9363-9367 (1995-09-26)
Normal somatic cells invariably enter a state of irreversibly arrested growth and altered function after a finite number of divisions. This process, termed replicative senescence, is thought to be a tumor-suppressive mechanism and an underlying cause of aging. There is
Mandy Oj Grootaert et al.
Autophagy, 11(11), 2014-2032 (2015-09-24)
Autophagy is triggered in vascular smooth muscle cells (VSMCs) of diseased arterial vessels. However, the role of VSMC autophagy in cardiovascular disease is poorly understood. Therefore, we investigated the effect of defective autophagy on VSMC survival and phenotype and its
Natalja E Fedorovich et al.
Biomaterials, 30(3), 344-353 (2008-10-22)
Photopolymerizable hydrogels, formed by UV-exposure of photosensitive polymers in the presence of photoinitiators, are widely used materials in tissue engineering research employed for cellular entrapment and patterning. During photopolymerization, the entrapped cells are directly exposed to polymer and photoinitiator molecules.
Phuc H Pham et al.
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