CBL1300

Anti-VCAM-1 Antibody, clone M/K

clone M/K - 2, Chemicon^®, from rat

• Synonim(y): CD106
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: M/K - 2, monoclonal
• Application: FACS, IHC, IP
• Citations: 8

Właściwości

• biological source: rat
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: M/K - 2, monoclonal
• species reactivity: mouse
• manufacturer/tradename: Chemicon^®
• technique(s): flow cytometry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable
• isotype: IgG1κ
• NCBI accession no.: NM_001078.2, NM_080682.1
• UniProt accession no.: P19320
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... L1CAM(3897), VCAM1(7412)

Opis

Application

Anti-VCAM-1 Antibody, clone M/K is an antibody against VCAM-1 for use in FC, IH & IP.

Studies of cell-cell interaction

Cell adhesion assays

Inhibition of lymphopoieisis in Whitlock-Witte long-term marrow cultures

Immunoprecipitation of the VCAM-1 integrin complex

Detection of VCAM-1 cells by immunofluorescence and immunohistochemical staining: acetone or fresh frozen sections work best; although zinc formalin or PLP fixations are reported to work but they have not be verified by Chemicon.

Optimal working dilutions must be determined by the end user.

Biochem/physiol Actions

Recognizes Murine vascular adhesion molecule (VCAM-1), single chain glycoprotein of Mr 100- kDa (under reducing conditions). VCAM-1 is present on the +/+ 2.4 stromal cell clone, large cells in bone marrow, and a subset of thymic macrophages. The stromal cell antigen is also detectable on the EOMA and BMS2 endothelial cell lines. The antibody reacts with all known isoforms of murine VCAM-1.

Physical form

Format: Purified

Analysis Note

Control
EOMA and BMS2 endothelial cell lysates

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Replaces: MAB2242

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Informacja o środkach bezpieczeństwa

• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• Klasa składowania: 12 - Non Combustible Liquids