AP112B
Goat Anti-Human IgG Antibody, biotin conjugate
Chemicon®, from goat
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About This Item
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.46
Polecane produkty
pochodzenie biologiczne
goat
Poziom jakości
białko sprzężone
biotin conjugate
forma przeciwciała
affinity purified immunoglobulin
rodzaj przeciwciała
secondary antibodies
klon
polyclonal
reaktywność gatunkowa
human
producent / nazwa handlowa
Chemicon®
metody
ELISA: suitable
western blot: suitable
Warunki transportu
wet ice
docelowa modyfikacja potranslacyjna
unmodified
Powiązane kategorie
Opis ogólny
IgG is a monomeric immunoglobulin, consists of two heavy chains (gamma chains) and two light chains. Each molecule has two antigen binding sites. IgG is the most abundant immunoglobulin. It is approximately equally distributed in blood and in interstitial fluid, constituting 75% of serum immunoglobulins in humans. This is the only isotype that can pass through the human placenta, providing protection to the fetus in its first weeks of life before its own immune system has developed. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%).
Specyficzność
The antibody reacts with the heavy chains of human IgG and with the light chains common to most human immunoglobulins.
Immunogen
Prepared from purified human IgG.
Zastosowanie
EIA: 1:20,000-1:400,000 using enzyme-streptavidin conjugate.
Western blots: 1:20,000-1:400,000 using enzyme-streptavidin conjugate.
Immunohistochemistry: 1:500-1:5,000 using enzyme-streptavidin conjugate.
Flow cytometry: 1:200-1:1,000.
Fluorescence Immunohisto/cytochemistry: 1:200-1:1,1000.
Optimal working dilutions must be determined by the end user.
Western blots: 1:20,000-1:400,000 using enzyme-streptavidin conjugate.
Immunohistochemistry: 1:500-1:5,000 using enzyme-streptavidin conjugate.
Flow cytometry: 1:200-1:1,000.
Fluorescence Immunohisto/cytochemistry: 1:200-1:1,1000.
Optimal working dilutions must be determined by the end user.
Research Category
Secondary & Control Antibodies
Secondary & Control Antibodies
Research Sub Category
Whole Immunoglobulin Secondary Antibodies
Whole Immunoglobulin Secondary Antibodies
This Goat anti-Human IgG Antibody, biotin conjugate is validated for use in ELISA, WB for the detection of Human IgG.
Postać fizyczna
ImmunoAffinity Purified
Purified by immunoaffinity chromatography using antigen coupled to agarose beads. Lyophilized. Buffer is 0.01 M Sodium Phosphate, 0.25 M NaCl, pH 7.6 with 15 mg/mL BSA, and 0.05% sodium azide.
Przechowywanie i stabilność
Maintain lyophilized product at 2°-8°C for up to 12 months from date of receipt. After reconstitution the product is stable for six weeks at 2°-8°C as an undiluted liquid. Reconstitute vial with 2 mL of distilled water. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of antibody (and buffer salts) will decrease to one-half of the original after the addition of glycerol and final working dilutions must be adjusted accordingly. Avoid repeated freeze thaw cycles.
Informacje prawne
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Hasło ostrzegawcze
Warning
Zwroty wskazujące rodzaj zagrożenia
Zwroty wskazujące środki ostrożności
Klasyfikacja zagrożeń
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Aquatic Chronic 3
Kod klasy składowania
11 - Combustible Solids
Klasa zagrożenia wodnego (WGK)
WGK 3
Certyfikaty analizy (CoA)
Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.
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