ABN904

Anti-GAD65/67 Antibody

rabbit polyclonal

• Synonim(y): Glutamate decarboxylase 2/Glutamate decarboxylase 1, 65 kDa/67kDa glutamic acid decarboxylase, GAD-65/67, Glutamate decarboxylase 65/67 kDa isoform
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• Clone: polyclonal
• Species reactivity: rat, mouse, human
• Application: IHC, WB
• Citations: 34

Właściwości

• Nazwa produktu: Anti-GAD 65/67, from rabbit, purified by affinity chromatography
• biological source: rabbit
• Quality Segment: 100
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: rat, mouse, human
• packaging: antibody small pack of 25 μg
• technique(s): immunohistochemistry: suitable (paraffin), western blot: suitable
• isotype: IgG
• NCBI accession no.: NP_000808.2, NP_000809
• UniProt accession no.: Q05329, Q99259
• shipped in: ambient
• target post-translational modification: unmodified
• Gene Information: human ... GAD1(2571), GAD2(2572)

Opis

General description

Glutamate decarboxylase 2/Glutamate decarboxylase 1 (UniProt: Q05329/Q99259; also known as 65 kDa/67kDa glutamic acid decarboxylase, GAD-65/67, Glutamate decarboxylase 65/67 kDa isoform) is encoded by GAD2/GAD1 (also known as GAD65/GAD67) gene (Gene ID: 2572/2571) in human. Glutamate decarboxylase isoforms are expressed in the brain where it is involved in gamma-aminobutyric acid (GABA) synthesis. Glutamate decarboxylase 2 is also expressed in pancreatic tissue. Both GAD65 and GAD67 synthesize GABA at different locations in the cell and at different developmental stages. Usually, GAD1 is distributed evenly throughout the cell while isoform GAD2 is mainly localized to nerve terminals. GAD1 synthesizes GABA for neuronal activity that is related to as synaptogenesis and protection from neural injury. However, GAD2 isoform synthesizes GABA for neurotransmission activity.

~60/62kDa observed; 65.41/66.90 kDa calculated, respectively for GAD2 and GAD1. Uncharacterized bands may be observed in some lysate(s).

Immunogen

Epitope: C-terminus

KLH-conjugated linear peptide corresponding to 14 amino acids from the C-terminal region of human glutamate decarboxylase 2 (GAD2).

Application

Anti-GAD 65/67 Antibody, Cat. No. ABN904, is a highly specific rabbit polyclonal antibody that targets Human, Mouse, and Rat GAD2 and GAD1 and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected GAD 65/67 in human cerebellum, mouse cerebral cortex, and rat cerebral cortex tissues.

Research Category
Neuroscience

Biochem/physiol Actions

This rabbit polyclonal antibody detects GAD2 and GAD1 in human, Mouse, and Rat. It targets an epitope within 14 amino acids from the C-terminal region.

Physical form

Affinity Purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Evaluated by Western Blotting in human brain tissue lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected GAD 65/67 in 10 µg of human brain tissue lysate.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Klasa składowania: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: does not flash
• flash_point_c: does not flash