AB9610 is a purified rabbit polyclonal antibody, so it is rabbit IgG.
AB9610
Anti-OLIG2 Antibody
CHEMICON®, rabbit polyclonal
Synonim(y):
Oligodendrocyte transcription factor 2, Oligo2, Class B basic helix-loop-helix protein 1, bHLHb1, Class E basic helix-loop-helix protein 19, bHLHe19, Protein kinase C-binding protein 2, Protein kinase C-binding protein RACK17
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| Gabaryty przesyłki | SKU | Dostępność | Cena netto |
|---|---|---|---|
| 100 μL | Przewidywany termin wysyłki23 marca 2026zKuehne + Nagel Sp. z o.o. | 2440,00 zł |
Informacje o tej pozycji
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Clone:
polyclonal
Species reactivity:
mouse, human, rat
Application:
ICC, IHC (p), IP, WB
Citations:
965
2440,00 zł
Przewidywany termin wysyłki23 marca 2026Szczegóły
Rekombinowane, niezawierające konserwantów przeciwciało jest dostępne dla Twojego celu. Wypróbuj ZRB1436
Pomoc techniczna
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Pozwól nam pomócNazwa produktu
Anti-Olig-2 Antibody, Chemicon®, from rabbit
biological source
rabbit
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
polyclonal
species reactivity
mouse, human, rat
manufacturer/tradename
Chemicon®
technique(s)
immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, immunoprecipitation (IP): suitable, western blot: suitable
NCBI accession no.
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General description
Oligodendrocyte transcription factor 2 (UniProt: Q13516, also known as Oligo2, Class B basic helix-loop-helix protein 1, bHLHb1, Class E basic helix-loop-helix protein 19, bHLHe19, Protein kinase C-binding protein 2, Protein kinase C-binding protein RACK17) is encoded by the OLIG2 (also known as BHLHB1, BHLHE19, PRKCBP2, RACK17) gene (Gene ID: 10215) in human. Olig2 is expressed in the brain in oligodendrocytes. Its strong expression is also observed in oligodendrogliomas. Olig2 is required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. It functions together with ZNF488 to promote oligodendrocyte differentiation. Olig2 is observed in both nucleus and the cytoplasm. Its nuclear localization signal is contained in the bHLH domain (aa 108-162). It can be masked in the native form and translocation to the nucleus may be mediated by interaction either with class E bHLH partner protein or with NKX2-2. Olig2 cooperates with Olig1 to establish the motor neuron progenitor domain of the embryonic neural tube. Removal of Cdx transcription factors is shown to result in the continued induction of Olig2 and ectopic production of cranial motor neuron progenitors. A chromosomal aberration involving Olig2 is shown to cause a form of T-cell acute lymphoblastic leukemia (T-ALL). (Ref.: Metzis, V., et al. (2018). Cell 175(4); 1105-1118).
~32 kDa
Immunogen
Recombinant mouse Olig-2
Application
Anti-Olig-2 Antibody is an antibody against Oligodendrocyte transcription factor 2 for use in IC, IH, IH(P), IP and WB.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neuronal & Glial Markers
Neuronal & Glial Markers
Western Blot Analysis:
1:2,500-1:5,000 dilution of a previous lot was used in western blot on human, rat and mouse cell lines (human oligodendroglioma, rat primary neurepithelial, mouse transfected) and tissues (brain and spinal cord).
Immunoprecipitation:
1:500-1:1,000 with human oligodendroglioma and mouse transfected cell line.
Optimal working dilutions must be determined by the end user.
Immunocytochemistry:
1:500-1:1,000 dilution of a previous lot was used in immunocytochemistry on human, rat and mouse cell lines (human oligodendroglioma, rat primary neurepithelial, mouse transfected) and tissues (brain and spinal cord).
Optimal working dilutions must be determined by the end user
1:2,500-1:5,000 dilution of a previous lot was used in western blot on human, rat and mouse cell lines (human oligodendroglioma, rat primary neurepithelial, mouse transfected) and tissues (brain and spinal cord).
Immunoprecipitation:
1:500-1:1,000 with human oligodendroglioma and mouse transfected cell line.
Optimal working dilutions must be determined by the end user.
Immunocytochemistry:
1:500-1:1,000 dilution of a previous lot was used in immunocytochemistry on human, rat and mouse cell lines (human oligodendroglioma, rat primary neurepithelial, mouse transfected) and tissues (brain and spinal cord).
Optimal working dilutions must be determined by the end user
Biochem/physiol Actions
Other species have not been tested.
Recognizes the ~32 kDa Olig-2 protein by Western blot.
Physical form
Ammonium Sulfate Precipitation
Format: Purified
Purified rabbit polyclonal. Liquid in 100 mM Glycine, 200 mM Tris, pH 8.0.
Preparation Note
Stable for 6 months at 2-8ºC from date of receipt.
Analysis Note
Control
Mouse brain whole cell lysate, PC12 whole cell lysate, brain (rat) tissue lysate
Mouse brain whole cell lysate, PC12 whole cell lysate, brain (rat) tissue lysate
Evaluated by immunohistochemistry on glioblastoma
Immunohistochemistry (Paraffin):
Representative lot data.
Anti-Olig2 (AB9610) staining pattern morphology in glioblastoma. Tissue was pretreated with TE Buffer, pH 9.0. Polyclonal antibody was diluted to 1:500, using IHC-Select Detection with HRP-DAB.
Optimal Staining With TE Buffer Epitope Retrieval: Glioblastoma
Immunohistochemistry (Paraffin):
Representative lot data.
Anti-Olig2 (AB9610) staining pattern morphology in glioblastoma. Tissue was pretreated with TE Buffer, pH 9.0. Polyclonal antibody was diluted to 1:500, using IHC-Select Detection with HRP-DAB.
Optimal Staining With TE Buffer Epitope Retrieval: Glioblastoma
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Klasa składowania
12 - Non Combustible Liquids
wgk
WGK 1
Certyfikaty analizy (CoA)
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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
PCP effector proteins inturned and fuzzy play nonredundant roles in the patterning but not convergent extension of mammalian neural tube.
Heydeck W, Liu A.
Developmental Dynamics null
Inga Nazarenko et al.
PloS one, 6(4), e18303-e18303 (2011-04-15)
Deregulation of platelet-derived growth factor (PDGF) signaling is a hallmark of malignant glioma. Two alternatively spliced PDGF-A mRNAs have been described, corresponding to a long (L) and a short (S) isoform of PDGF-A. In contrast to PDGF-A(S), the PDGF-A(L) isoform
The probable cell of origin of NF1- and PDGF-driven glioblastomas.
Hambardzumyan, D; Cheng, YK; Haeno, H; Holland, EC; Michor, F
Testing null
Loss of ATM/Chk2/p53 pathway components accelerates tumor development and contributes to radiation resistance in gliomas.
Squatrito, M; Brennan, CW; Helmy, K; Huse, JT; Petrini, JH; Holland, EC
Cancer Cell null
Dominik Sturm et al.
Cancer cell, 22(4), 425-437 (2012-10-20)
Glioblastoma (GBM) is a brain tumor that carries a dismal prognosis and displays considerable heterogeneity. We have recently identified recurrent H3F3A mutations affecting two critical amino acids (K27 and G34) of histone H3.3 in one-third of pediatric GBM. Here, we
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Numer pozycji handlu globalnego
| SKU | NUMER GTIN |
|---|---|
| AB9610 | 04053252366536 |
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Bonjour, il s'agit d'un anticorps IgM ou IgG ? pour le choix du secondaire.
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