AB1286-I

Anti-Monocarboxylate Transporter 1 Antibody

from chicken, purified by affinity chromatography

• Synonim(y): Protein IGO1
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: polyclonal
• Application: IHC, WB
• Citations: 24

Właściwości

• biological source: chicken
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: affinity purified immunoglobulin
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: rat
• species reactivity (predicted by homology): mouse (immunogen homology)
• technique(s): immunohistochemistry: suitable (paraffin), western blot: suitable
• isotype: IgY
• NCBI accession no.: NP_036848
• UniProt accession no.: P53987
• shipped in: wet ice
• target post-translational modification: unmodified

Opis

General description

Bands are observed at ~44 kDa and ~55 kDa This protein has been observed at ~47 kDa (Sepponen, K., et al. (2003). Acta Physiol Scand. 177(1):79-86.). In addition this protein has been observed at ~55 kDa in certain cell lysates, except in the rumen where two distinct bands appear at ~47 kDa and ~55 kDa (Koho, N., et al. (2005). Comp Biochem Physiol A Mol Integr Physiol. 141(1):29-34.). It is also known to be glycosylated (Carpenter, L., et al. (1996). Biochim Biophys Acta. 1279(2):157-163).

Monocarboxylate Transporter 1 (MCT1), also known as Solute carrier family 16 member 1 (SLC16A1), catalyzes the rapid transport across the plasma membrane of many monocarboxylates such as lactate, pyruvate, branched-chain oxo acids derived from leucine, valine and isoleucine, and the ketone bodies acetoacetate, beta-hydroxybutyrate and acetate. Monocarboxylate Transporter 1 is located in the cell membrane, and is widely expressed in both normal and cancer cells. Monocarboxylate Transporter 1 is involved in symptomatic deficiency in lactate transport (SDLT).

Immunogen

Epitope: C-terminus

KLH-conjugated linear peptide corresponding to the C-terminus of rat Monocarboxylate Transporter 1.

Application

Anti-Monocarboxylate Transporter 1 Antibody is a highly specific chicken polyclonal antibody, that targets MCT1 & has been tested in western blotting & IHC (Paraffin).

Immunohistochemistry Analysis: A 1:2,500 dilution from a representative lot detected rat skeletal muscle and rat renal cortex tissues.

Research Category
Neuroscience

Research Sub Category
Signaling Neuroscience

Biochem/physiol Actions

This antibody recognizes the C-terminus of Monocarboxylate Transporter 1.

Physical form

Affinity purified

Purified chicken polyclonal IgY in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Rat brain membrane tissue lysate

Evaluated by Western Blot in rat brain membrane tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected rat brain membrane tissue lysate in 10 µg of rat brain membrane tissue lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Replaces: AB1286

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Klasa składowania: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable