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BugBuster® Master Mix

Synonim(y):

BugBuster® Protein Extraction Reagent with Benzonase® Nuclease and rLysozyme solution, Cell Lysis Master Mix

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Gabaryty przesyłkiSKUDostępnośćCena netto
100 mL

Przewidywany termin wysyłki11 maja 2026zKuehne + Nagel Sp. z o.o.

1400,00 zł
500 mL

Przewidywany termin wysyłki19 maja 2026zKuehne + Nagel Sp. z o.o.

4520,00 zł

Informacje o tej pozycji

NACRES:
NA.32
UNSPSC Code:
41106511

1400,00 zł

Przewidywany termin wysyłki11 maja 2026Szczegóły

​
Pomoc techniczna
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form

liquid

Quality Level

200

manufacturer/tradename

Novagen®

storage condition

OK to freeze

shipped in

wet ice

storage temp.

2-8°C

General description

The BugBuster® Master Mix enables efficient protein purification and other related applications. It replaces the need for mechanical methods like the French press or sonication. The two available package sizes contain sufficient reagents for extracting proteins from either 20 grams or 100 grams of cell paste.

Application

BugBuster® Master Mix has been used:
  • to resuspend and lyse the cell pellets during protein purification
  • to extract proteins for immunoblotting
  • as a bacterial lysis buffer for the preparation of inclusion bodies


    

Biochem/physiol Actions

BugBuster® Protein Extraction Reagent is specifically designed to gently disrupt the cell wall of E. coli, allowing the release of soluble proteins. This unique formulation utilizes a detergent mix that can effectively perforate the cell wall without denaturing the soluble proteins. By combining BugBuster Reagent, rLysozyme solution, and Benzonase Nuclease, our BugBuster Master Mix enhances protein extraction efficiency and simplifies downstream processing of protein extracts.

Features and Benefits

  • Efficient lysis of Gram-positive and Gram-negative bacteria
  • Maximum recovery of active, soluble protein
  • Capable of cell wall perforation without denaturing soluble protein
  • Useful for the preparation of high purity inclusion bodies
  • Compatible with protease and phosphatase inhibitor cocktail

  • Offers a convenient, fast, and cost-effective solution for releasing the expressed target protein.
  • The Master Mix eliminates the need to dilute or perform separate addition steps.

Legal Information

BUGBUSTER is a registered trademark of Merck KGaA, Darmstadt, Germany
Benzonase is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)
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form

liquid

form

liquid

form

liquid

form

liquid

storage condition

OK to freeze

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

storage condition

OK to freeze

Quality Level

200

Quality Level

100

Quality Level

100

Quality Level

-

shipped in

wet ice

shipped in

ambient

shipped in

wet ice

shipped in

wet ice

manufacturer/tradename

Novagen®

manufacturer/tradename

Novagen®

manufacturer/tradename

Novagen®

manufacturer/tradename

Novagen®

storage temp.

2-8°C

storage temp.

10-30°C

storage temp.

2-8°C

storage temp.

-

Klasa składowania

12 - Non Combustible Liquids

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Produkty

Citation Spotlight: BugBuster® Protein Extraction Reagent for Efficient Protein Extraction from Bacterial Pathogens

Citation Spotlight: BugBuster® Protein Extraction Reagent for Efficient Protein Extraction from Bacterial Pathogens

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