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69041

B834(DE3) Competent Cells - Novagen

Escherichia coli, rod shaped

Synonim(y):

Competent cells

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Gabaryty przesyłkiSKUDostępnośćCena netto
0.4 mL

Przewidywany termin wysyłki07 kwietnia 2026zKuehne + Nagel Sp. z o.o.

662,00 zł
1 mL

Przewidywany termin wysyłki07 kwietnia 2026zKuehne + Nagel Sp. z o.o.

1320,00 zł

Informacje o tej pozycji

UNSPSC Code:
41106202
NACRES:
NA.81
Biological source:
Escherichia coli
Growth mode:
adherent or suspension
Morphology:
rod shaped
Manufacturer/tradename:
Novagen®

662,00 zł

Przewidywany termin wysyłki07 kwietnia 2026Szczegóły

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Nazwa produktu

B834(DE3) Competent Cells - Novagen, B834 is the parental strain for BL21. These hosts are methionine auxotrophs and allow high specific activity labeling of target proteins with 35S-methionine and selenomethionine for crystallography.

biological source

Escherichia coli

manufacturer/tradename

Novagen®

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

growth mode

adherent or suspension

morphology

rod shaped

technique(s)

microbiological culture: suitable

cell transformation

transformation efficiency: >2×106 cfu/μg

shipped in

dry ice

storage temp.

−70°C

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Ta pozycja
69042-M6995670911
B834(DE3) Competent Cells - Novagen B834 is the parental strain for BL21. These hosts are methionine auxotrophs and allow high specific activity labeling of target proteins with 35S-methionine and selenomethionine for crystallography.

Sigma-Aldrich

69041

B834(DE3) Competent Cells - Novagen

B834(DE3)pLysS Competent Cells - Novagen B834 host strains are methionine auxotrophs that allows high specific activity labeling of target proteins with 35S-methionine. T7 lysozyme expression suppresses basal T7 expression.

Sigma-Aldrich

69042-M

B834(DE3)pLysS Competent Cells - Novagen

BLR(DE3)pLysS Competent Cells - Novagen BLR is a recA derivative of BL21 that improves plasmid monomer yields and may help stabilize target plasmids containing repetitive sequences. T7 lysozyme expression suppresses basal T7 expression.

Sigma-Aldrich

69956

BLR(DE3)pLysS Competent Cells - Novagen

Origami B Competent Cell Set - Novagen

Sigma-Aldrich

70911

Origami B Competent Cell Set - Novagen

growth mode

adherent or suspension

growth mode

adherent or suspension

growth mode

adherent or suspension

growth mode

adherent or suspension

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

biological source

Escherichia coli

biological source

Escherichia coli

biological source

Escherichia coli

biological source

Escherichia coli

morphology

rod shaped

morphology

rod shaped

morphology

rod shaped

morphology

rod shaped

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

storage temp.

−70°C

storage temp.

−70°C

storage temp.

−70°C

storage temp.

−70°C

General description

B834 is the parental strain for BL21 (1). These protease-deficient hosts are methionine auxotrophs and allow high specific-activity labeling of target proteins with 35S-methionine and selenomethionine for crystallography (2).

DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.
Genotype: F-ompT hsdSB(rB- mB-) gal dcm met (DE3)

This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges us to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
B834 is the parental strain for BL21. These hosts are methionine auxotrophs and allow high specific activity labeling of target proteins with 35S-methionine and selenomethionine for crystallography.

Other Notes

1. Wood, W. B. (1966) J. Mol. Biol. 16, 118–133.

2. Leahy, D. J., Hendrickson, W. A., Aukhil, I., and Erickson, H. P. (1992) Science 258, 987–991.
0.4 ml1 mlComponent

•2 × 0.2 ml5 × 0.2 mlB834(DE3)Competent Cells

•2 × 2 ml4 × 2 mlSOC Medium

•2 ng2 ngTest Plasmid

Legal Information

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Multiple Toxicity Values, refer to MSDS (O)
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Klasa składowania

10 - Combustible liquids

wgk

WGK 2

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Swi2/Snf2 ATPases remodel protein:DNA complexes in all of the fundamental chromosome-associated processes. The single-subunit remodeler Mot1 dissociates TATA box-binding protein (TBP):DNA complexes and provides a simple model for obtaining structural insights into the action of Swi2/Snf2 ATPases. Previously we reported
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Structure (London, England : 1993), 29(7), 731-742 (2021-03-20)
Branched Lipid II, required for the formation of indirectly crosslinked peptidoglycan, is generated by MurM, a protein essential for high-level penicillin resistance in the human pathogen Streptococcus pneumoniae. We have solved the X-ray crystal structure of Staphylococcus aureus FemX, an
Arabinda Nayak et al.
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The dicistrovirus, Cricket paralysis virus (CrPV) encodes an RNA interference (RNAi) suppressor, 1A, which modulates viral virulence. Using the Drosophila model, we combined structural, biochemical, and virological approaches to elucidate the strategies by which CrPV-1A restricts RNAi immunity. The atomic
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SKUNUMER GTIN
69041-304055977259711
69041-404055977259728

Questions

  1. Is this strain protease-free? If no what all proteases are present in it?

    1 answer

    1. This product is derived from a B strain deficient in Lon and OmpT proteases. B834, a protease-deficient host is a methionine auxotroph and allows high specific activity labeling of target proteins with 35S-methionine and selenomethionine for crystallography.

      Please see the link below to access the User Guide for this product:
      https://www.emdmillipore.com/US/en/product/B834DE3-Competent-Cells-Novagen,EMD_BIO-69041#anchor_USP

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