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Merck

574797

TAE Buffer, 10X, Molecular Biology Grade

A 10X concentrate that can be diluted to a 1X solution containing 40 mM Tris, 40 mM acetate, and 1 mM EDTA, pH ~8.3.

Synonim(y):

Tris-Acetate-EDTA Buffer

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574797-1L

342,00 zł

342,00 zł

Przewidywany termin wysyłki16 marca 2026Szczegóły

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Informacje o tej pozycji

NACRES:
NA.25
UNSPSC Code:
12161700

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form

liquid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze, desiccated

technique(s)

electrophoresis: suitable

foreign activity

DNase, RNase, protease, none detected

shipped in

ambient

storage temp.

15-25°C

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Ta pozycja
574793574795PPB008
TAE Buffer, 10X, Molecular Biology Grade A 10X concentrate that can be diluted to a 1X solution containing 40 mM Tris, 40 mM acetate, and 1 mM EDTA, pH ~8.3.

574797

TAE Buffer, 10X, Molecular Biology Grade

TE Buffer, 100X, Molecular Biology Grade A 100X concentrate that, when diluted to 1X, contains 10 mM Tris, 1 mM EDTA, pH ~8.0.

Sigma-Aldrich

574793

TE Buffer, 100X, Molecular Biology Grade

TBE Buffer, 10X, Molecular Biology Grade A 10X concentrate that can be diluted to a 1X solution containing 89 mM Tris, 89 mM boric acid, 2 mM EDTA, pH ~8.3.

574795

TBE Buffer, 10X, Molecular Biology Grade

Tris Acetate-EDTA buffer pH 8.3, pHast Pack™, powder

Sigma-Aldrich

PPB008

Tris Acetate-EDTA buffer

technique(s)

electrophoresis: suitable

technique(s)

-

technique(s)

-

technique(s)

-

form

liquid

form

liquid

form

liquid

form

powder

storage condition

OK to freeze, desiccated

storage condition

OK to freeze, desiccated

storage condition

OK to freeze

storage condition

-

storage temp.

15-25°C

storage temp.

15-25°C

storage temp.

15-25°C

storage temp.

-

foreign activity

DNase, RNase, protease, none detected

foreign activity

DNase, RNase, protease, none detected

foreign activity

DNase, RNase, protease, none detected

foreign activity

DNAse, none detected, Nickase, none detected, Protease, none detected, RNAse, none detected

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

manufacturer/tradename

-

General description

Tris, acetate, and ethylenediaminetetraacetic acid (TAE) buffer consists of tris(2-amino-2-[hydroxymethyl]-1,3-propanediol) base, acetate, and ethylenediaminetetraacetic acid (EDTA). This is one of the most used running buffers in nucleic acid electrophoresis. TAE buffer improves the separation or resolution of large DNA fragments.[1]TAE, a standard buffer, is mainly used to perform agarose gel electrophoresis of DNA and RNA in slab gels. TAE is one of the commonly used buffers in every biochemistry and molecular biology laboratory.

Application

TAE Buffer, 10X, Molecular Biology Grade - Calbiochem has been used in 1X concentration for the preparation of agarose gel and as a running buffer in gel electrophoresis.[2]

Preparation Note

Dilute 1:10 with H₂O to prepare a 1X solution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Irritant (B)
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pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H319

Hazard Classifications

Eye Irrit. 2

Klasa składowania

12 - Non Combustible Liquids

wgk

WGK 1

Certyfikaty analizy (CoA)

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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Modification of gel architecture and TBE/TAE buffer composition to minimize heating during agarose gel electrophoresis
Sanderson BA, et al.
Analytical Biochemistry, 454, 44-52 (2014)
Bin Li et al.
Nature protocols, 13(5), 899-914 (2018-04-07)
Cpf1, a CRISPR endonuclease discovered in Prevotella and Francisella 1 bacteria, offers an alternative platform for CRISPR-based genome editing beyond the commonly used CRISPR-Cas9 system originally discovered in Streptococcus pyogenes. This protocol enables the design of engineered CRISPR-Cpf1 components, both
Brian A Sanderson et al.
Analytical biochemistry, 454, 44-52 (2014-03-19)
Agarose gel electrophoresis of DNA and RNA is routinely performed using buffers containing either Tris, acetate, and EDTA (TAE) or Tris, borate, and EDTA (TBE). Gels are run at a low, constant voltage (∼10 V/cm) to minimize current and asymmetric

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