481001
Anti-Neutrophil Elastase Rabbit pAb
liquid, Calbiochem®
Synonim(y):
Neutrophil elastase antibody, Rabbit monoclonal antibody
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About This Item
Kod UNSPSC:
12352203
NACRES:
NA.43
Polecane produkty
pochodzenie biologiczne
rabbit
Poziom jakości
forma przeciwciała
purified antibody
rodzaj przeciwciała
primary antibodies
klon
polyclonal
Postać
liquid
zawiera
≤0.1% sodium azide as preservative
reaktywność gatunkowa
human
producent / nazwa handlowa
Calbiochem®
warunki przechowywania
OK to freeze
avoid repeated freeze/thaw cycles
izotyp
IgG
Warunki transportu
wet ice
temp. przechowywania
−20°C
docelowa modyfikacja potranslacyjna
unmodified
informacje o genach
human ... ELANE(1991)
Opis ogólny
Ammonium sulfate precipitated rabbit polyclonal antibody. Recognizes human neutrophil elastase.
Recognizes the neutrophil elastase protein.
This Anti-Neutrophil Elastase Rabbit pAb is validated for use in ELISA, Immunoblotting, Immunoelectrophoresis, Immunoprecipitation for the detection of Neutrophil Elastase.
Immunogen
Human
human neutrophil elastase
Zastosowanie
ELISA (≥ 1:1000)
Immunoblotting (see comments)
Immunoelectrophoresis (see comments)
Immunoprecipitation (see comments)
Immunoblotting (see comments)
Immunoelectrophoresis (see comments)
Immunoprecipitation (see comments)
Opakowanie
Please refer to vial label for lot-specific concentration.
Ostrzeżenie
Toxicity: Standard Handling (A)
Postać fizyczna
In PBS, pH 7.5.
Rekonstytucja
Following initial thaw, aliquot and freeze (-20°C).
Inne uwagi
Single arc by immunoelectrophoresis against neutrophil granule extract and human neutrophil elastase. This antibody has also been reported to work for immunoblotting and immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.
Informacje prawne
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Kod klasy składowania
12 - Non Combustible Liquids
Klasa zagrożenia wodnego (WGK)
nwg
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
Avinash Khandagale et al.
Journal of leukocyte biology, 104(6), 1199-1213 (2018-08-15)
Mutations in the gene JAGN1 were recently discovered in patients with severe congenital neutropenia (SCN). Neutrophils release neutrophil extracellular traps (NETs) consisting of decondensed chromatin decorated with various granular proteins such as neutrophil elastase and myeloperoxidase (MPO) to combat microbial
Elaine F Kenny et al.
eLife, 6 (2017-06-03)
Neutrophils release neutrophil extracellular traps (NETs) which ensnare pathogens and have pathogenic functions in diverse diseases. We examined the NETosis pathways induced by five stimuli; PMA, the calcium ionophore A23187, nigericin, Candida albicans and Group B Streptococcus. We studied NET
Dae-goon Yoo et al.
Journal of immunology (Baltimore, Md. : 1950), 192(10), 4728-4738 (2014-04-18)
Cystic fibrosis (CF) airways are characterized by bacterial infections, excess mucus production, and robust neutrophil recruitment. The main CF airway pathogen is Pseudomonas aeruginosa. Neutrophils are not capable of clearing the infection. Neutrophil primary granule components, myeloperoxidase (MPO) and human
Volker Brinkmann et al.
Frontiers in immunology, 3, 413-413 (2013-01-15)
Neutrophil Extracellular Traps (NETs) consist of decondensed chromatin studded with granular and some cytoplasmic proteins. They are formed by activated neutrophil granulocytes, also called polymorphonuclear leukocytes (PMN) as the result of an active cell death program, named NETosis. NETosis can
Frauke Pedersen et al.
Respiratory medicine, 109(10), 1360-1362 (2015-09-01)
Chronic obstructive pulmonary disease (COPD) is characterized by neutrophilic airway inflammation. Neutrophil extracellular trap (NET) formation - a meshwork of neutrophil DNA components and neutrophil enzymes are involved in innate immunity and inflammation. Little is known about the presence of
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