480716

α2-3,6,8,9-Neuraminidase, Arthrobacter ureafaciens, Recombinant, E. coli

• Synonim(y): α2-3,6,8,9-Neuraminidase, Arthrobacter ureafaciens, Recombinant, E. coli, N-Acetylneuraminyl Hydrolase, Sialidase
• Numer CAS: 9001-67-6
• Numer WE: 3.2.1.18 (BRENDA, IUBMB)
• NACRES: NA.54
• UNSPSC Code: 12352202
• MDL number: MFCD00040440
• Specific activity: ≥40 units/mg protein, ≥5 units/mL
• Recombinant: expressed in E. coli

Właściwości

• recombinant: expressed in E. coli
• Quality Segment: 100
• description: RTECS - QQ3450000
• form: liquid
• specific activity: ≥40 units/mg protein, ≥5 units/mL
• manufacturer/tradename: Calbiochem^®
• storage condition: do not freeze
• foreign activity: N-acetylglucosaminidase, α-fucosidase, α- and β-galactosidase, α- and β-mannosidase, proteases, none detected
• shipped in: wet ice
• storage temp.: 2-8°C

Opis

General description

Note: 1 mU = 1 milliunit.

Recombinant, Arthrobacter ureafaciens α2-3,6,8,9-Neuraminidase expressed in E. coli. Catalyzes the hydrolysis of all non-reducing terminal sialic acid residues from complex carbohydrates and glycoproteins. High concentrations of this sialidase and prolonged periods of incubation result in the additional cleavage of branched sialic acids. This enzyme is found as two active species of MW 60000 and 69000.

Physical form

In 20 mM Tris-HCl, 20 mM NaCl, pH 7.5.

Other Notes

Means, R.E. and Desrosiers, R.C. 2000. J. Virol.74, 11181.
Prime, S., et al. 1996. J. Chromatogr. A. 720, 263.
Roggentin, P., et al. 1995. Biol. Chem. Hoppe Seyler 376, 569
Dwek, R.A., et al. 1993. Annu. Rev. Biochem. 62, 65.

One unit is defined as the amount of enzyme that will catalyze the release of 1 µmol of methylumbelliferone from 2ʹ-(4-methylumbelliferyl)-α-D-N-acetylneuraminic acid per min at 37°C, pH 5.0. (Note: 1 mU = 1 milliunit).

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Harmful (C)

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Informacja o środkach bezpieczeństwa

• Klasa składowania: 12 - Non Combustible Liquids
• wgk: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable