The volume may be determined using the lot specific Enzymatic Activity reported in the Certificate of Analysis. For example, if a specific lot reports an Enzymatic Activity of 7.17 U/mL, the 1 unit pack size will have a volume of roughly 139 uL and the 2 unit pack size would have a volume of 278 uL. Please see the link below to review a sample or lot specific Certificate of Analysis:
https://www.sigmaaldrich.com/US/en/product/sigma/n7885#product-documentation
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N7885
Neuraminidase from Vibrio cholerae
Type III, buffered aqueous solution, 0.2 μm filtered, 1-5 units/mg protein (Lowry, using NAN-lactose)
Synonim(y):
Acyl-neuraminyl Hydrolase, Receptor-destroying enzyme, Sialidase
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| Gabaryty przesyłki | SKU | Dostępność | Cena netto |
|---|---|---|---|
| 1 unit | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 767,00 zł | |
| 2 units | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 1420,00 zł |
Informacje o tej pozycji
Numer CAS:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-624-6
MDL number:
Specific activity:
1-5 units/mg protein (Lowry, using NAN-lactose)
767,00 zł
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
Pomoc techniczna
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Pozwól nam pomócQuality Segment
type
Type III
form
buffered aqueous solution
specific activity
1-5 units/mg protein (Lowry, using NAN-lactose)
mol wt
83 kDa
application(s)
diagnostic assay manufacturing
foreign activity
Protease and NAN-aldolase, present
storage temp.
2-8°C
General description
Neuraminidase enzymes are glycoside hydrolase enzymes that catalyze hydrolysis of terminal sialic acid residues. The most well-known are the viral nearamidases, which promote influenza virus release.
Application
Neuraminidase from Vibrio cholerae has been used in a study to assess its role in the binding and uptake of cholera toxin by susceptible cells. [1] It has also been used in a study to investigate the preparation of a sodium substrate and its use in a fluorometric assay of neuraminidase. [2]
Neurminidase is used as a cell-surface probe for glycoconjugate distribution and in substrate specificity studies.
Biochem/physiol Actions
Vibrio cholerae neuraminidase has been shown to promote cholera toxin infection by binding the toxin and aiding in its uptake by cells. [1]
Physical form
Aqueous solution, pH 5.5, containing 0.15 M NaCl and 4 mM CaCl2.
Preparation Note
Chromatographically purified.
Analysis Note
Preservative free.
Other Notes
One unit will liberate 1.0 μmole of N-acetylneuraminic acid per min at pH 5.0 at 37 °C using NAN-lactose or bovine submaxillary mucin, unless otherwise specified. Prices based on units using NAN-lactose as substrate.
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Ta pozycja | |||
|---|---|---|---|
| specific activity 1-5 units/mg protein (Lowry, using NAN-lactose) | specific activity 8-24 units/mg protein (Lowry, using NAN-lactose) | specific activity ≥0.1 units/mg solid (using mucin), ≥1.3 units/mg solid (using 4MU-NANA) | specific activity ≥50 units/mg protein (using 4MU-NANA) |
| application(s) diagnostic assay manufacturing | application(s) - | application(s) diagnostic assay manufacturing | application(s) - |
| form buffered aqueous solution | form buffered aqueous solution | form lyophilized powder | form lyophilized powder |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C |
| mol wt 83 kDa | mol wt - | mol wt - | mol wt - |
| foreign activity Protease and NAN-aldolase, present | foreign activity Protease and NAN-aldolase, present | foreign activity Protease and NAN-aldolase, present | foreign activity - |
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signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Klasa składowania
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Protokoły
Enzymatic Assay of Neuraminidase applies to products that have a specification for neuraminidase content by enzymatic determination.
Produkty
Understand sialic acid structure, function, signaling, and modifications. Easily find products for sialic acid research.
Powiązane treści
Instructions
Numer pozycji handlu globalnego
| SKU | NUMER GTIN |
|---|---|
| N7885-1UN | 04061834118282 |
| N7885-2UN | 04061834118299 |
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Dear Customer service I have a question regarding the product (N7885 Neuraminidase from Vibrio cholerae). In how much volume does the 1 Unit or 2 Unit package arrive? Could you provide a volume (or mg if it's powder)? Thanks, Ben
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