420320

KT5720

≥95% (HPLC), solid, protein kinase A inhibitor, Calbiochem^®

• Synonim(y): KT5720, PKA Inhibitor VII
• Wzór empiryczny (zapis Hilla): C₃₂H₃₁N₃O₅
• Numer CAS: 108068-98-0
• Masa cząsteczkowa: 537.61
• UNSPSC Code: 12352200
• NACRES: NA.77
• MDL number: MFCD00132118
• Assay: ≥95% (HPLC)
• Form: solid
• Quality level: 100
• Storage condition: OK to freeze, protect from light

Właściwości

• Nazwa produktu: KT5720, A potent, specific, cell-permeable, reversible and ATP-competitive inhibitor of protein kinase A (K_i = 56 nM) that is prepared by a chemical modification of K-252a.
• Quality Level: 100
• assay: ≥95% (HPLC)
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, protect from light
• color: white
• solubility: DMSO: 1 mg/mL
• shipped in: ambient
• storage temp.: −20°C
• SMILES string: [n]21c3c4[n](c6c(c4c7c(c3c8c2cccc8)CNC7=O)cccc6)[C@H]5O[C@@]1([C@](C5)(O)C(=O)OCCCCCC)C
• InChI: 1S/C32H31N3O5/c1-3-4-5-10-15-39-30(37)32(38)16-23-34-21-13-8-6-11-18(21)25-26-20(17-33-29(26)36)24-19-12-7-9-14-22(19)35(28(24)27(25)34)31(32,2)40-23/h6-9,11-14,23,38H,3-5,10,15-17H2,1-2H3,(H,33,36)/t23-,31+,32+/m0/s1
• InChI key: ZHEHVZXPFVXKEY-RUAOOFDTSA-N

Opis

General description

A potent, specific, cell-permeable, reversible and ATP-competitive inhibitor of protein kinase A (K_i = 56 nM) that is prepared by a chemical modification of K-252a. Does not significantly affect the activities of PKC, PKG, and MLCK. Inhibits axon branching in cultured neurons. A 2 mM (50 µg/47 µl) solution of KT 5720 (Cat. No. 420323) in DMSO is also available.

Prepared by a chemical modification of K-252a. A potent, specific, cell-permeable, reversible and ATP-competitive inhibitor of protein kinase A (K_i = 56 nM). Does not significantly affect the activity of PKC, PKG, or MLCK. Inhibits axon branching in cultured neurons.

Biochem/physiol Actions

Cell permeable: yes

Primary Target
PKA

Product competes with ATP.

Reversible: yes

Target K_i: 56 nM for protein kinase A

Preparation Note

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Further dilute with aqueous buffers just prior to use.

Other Notes

Simpson, C.S., and Morris, B.J. 1995. Neuroscience 68, 97.
Cabell, L., and Audesirk, G. 1993. Int. J. Dev. Neurosci.11, 357.
Gadbois, D.M., et al. 1992. Proc. Natl. Acad. Sci. USA89, 8626.
Kase, H., et al. 1987. Biochem. Biophys. Res. Commun.142, 436.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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Informacja o środkach bezpieczeństwa

• Klasa składowania: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable